LOCUS       sequence-131213-        8078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3G, isoform z protein (truncated at cleavage site 
            P1; ie, short), C-terminally fused to alkaline phosphatase + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3g.z(S)-AP-His
FEATURES             Location/Qualifiers
     source          1..8078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4658..4675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4701..4822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4738..4757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4792..4811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4860..4877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5031..5619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5111..5130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5790..6581,6582..6650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6413..6432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6651..6755)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6823..6841
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6879..6901)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6903..6923)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6908..7238
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7089..7224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7151..7170
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7375..7394
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7461..7916)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7598..7619)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7810..7829
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8042..8064
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8056..8073
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8057..8073
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggacccatc
     1621 tgcgtgggcc atctgctgcc tcctggggag ccttctgttc catgtgggca tccccagccc
     1681 tggtcccagc cccagtgtgc ctcgtctgcg gctctcctac agagatctcc tgtctaccaa
     1741 ccgctctgcc atctttctgg gcccacgggg ttccctagac ctccaagtca tgtacctgga
     1801 tgagtatcga gatcgcctct ttctgggaag ccgagatgcc ctttactctc tgagactgga
     1861 tcaggcatgg ccagaccccc gggaggtctt gtggctgcca cagccaggac agaaggtgga
     1921 atgtgtccgc aagggaaaag accctttgac ggagtgtgcc aacttcgtgc gggtcttaca
     1981 accccataac cggacccatc tgctggcctg tggcacagga gccttccaac ccatctgcac
     2041 cttcatcaca gtggggcatc gaggggagca tgtgctccgc ttggatgcta gcagcgtcga
     2101 gaatggaagg gggcgttgcc cacatgagcc cagccgaccc ttcgccagca cctttgtagg
     2161 tggggagcta tacacaggtc tcactgccga tttcctggga cgtgaggcca tgattttccg
     2221 gagtgggggt ccccgaccag ccctgcgttc tgactctgac cagagcctcc tacatgaacc
     2281 ccggtttgtg atggctgctc ggatcccaga taactctgac cgggacgacg acaaggtgta
     2341 cttcttcttc tccgagactg tcccgtcacc agatggtggc ccaggtcatg tcaccatcag
     2401 ccgagtgggc cgcgtctgtg tgaatgatgc tggtggccag cgggtgctgg tgaacaaatg
     2461 gagcactttc ctcaaggccc ggctggtgtg ctctgtgcct ggcccgggtg gggctgagac
     2521 tcactttgac cagctagagg atgtgttcct gctgtggccc aaggccggga agagcctcga
     2581 ggtgtatgcg ctgttcagca ctgtcagtgc tgtgttccag ggctttgctg tctgtgtgta
     2641 ccacatggta gacatctggg aggtcttcaa tgggcccttt gcccaccgag atggccctca
     2701 gcatcagtgg ggaccctatg ggggcaaggt gcccttccct cgccccggtg tgcctgtgag
     2761 gcctcggcgt ggccgccctg tcctggtcaa aactcacttg gctcagcgac tgcgccagat
     2821 tgtggtggat cgagtggagg ctgaggatgg gacctatgat gtcatcttcc tagggactga
     2881 ttcgggttct gtgctcaaag tcattgccct ccagggtggt ggcttgactg aacctgaaga
     2941 ggtggttttg gaggagctcc aggtgtttaa ggtgccaacg cccatcactg agatggagat
     3001 ctctgtcaaa aggcaaacgc tgtatgtggg ctctccactg ggtgtggccc ggctgcagct
     3061 gcaccagtgt gagacttacg gcagtgcctg tgctgagtgc tgcctggccc gggacccata
     3121 ctgtgcttgg gatggcactg cctgtgcccg ctaccgcccc agctctggcg gcgcgcctgg
     3181 atcttccgga atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc
     3241 cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat
     3301 catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg
     3361 gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt
     3421 ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac
     3481 ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg
     3541 ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa
     3601 gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc
     3661 cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc
     3721 ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga
     3781 cgtgatccta ggtgggggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta
     3841 cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg
     3901 gctggcgaag cgccagggtg cccggtacgt gtggaaccgc actgagctca tgcaggcttc
     3961 cctggacccg tctgtggccc atctcatggg tctctttgag cctggagaca tgaaatacga
     4021 gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg
     4081 cctgctgagc aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca
     4141 tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc
     4201 cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga
     4261 ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct
     4321 ggcccctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc
     4381 aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc
     4441 cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt
     4501 ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt
     4561 catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc
     4621 gccccccgcc ggcaccaccg acgccgcgca cccgggtcat catcaccatc accattaatc
     4681 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     4741 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4801 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     4861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5461 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5701 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5761 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5821 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5881 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5941 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6001 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6061 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6121 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6181 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6241 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6301 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6361 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6421 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6481 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6541 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6601 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6661 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6721 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6781 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6841 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6901 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     6961 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7021 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7081 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7141 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7201 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7261 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7321 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     7381 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     7441 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     7501 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7561 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7621 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7681 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7741 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7801 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7861 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     7921 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7981 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8041 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
