LOCUS       sequence-13123-e        3624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1683-HQ858767
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1181..1280)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1297..1316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1298..1316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1321..1337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1321..1337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1439..1458)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1450..2259
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1517..1536)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2406..2994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2895..2914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3148..3165
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3324..3351)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3443..3529)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3578..3600
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3592..3609
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3593..3609
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgttctcc ggcaccggac cctccggcgg ggcagcccac
      181 tctacgtcca ccggtggtgg gggtggctcg ccttccgaca ggggcggccc cgcgccctcg
      241 gcctcggcgt cggtctccac gccagccagc gagagcaccg tcgcccgccg cctaaacgga
      301 cttgaccccc acggcgacga cgctccatcg tcgcagccag ccacgagcaa gaagaaaaag
      361 agaggggcac gagcagttgg tcctgacaaa aatggccgtg gattgcgtca atttagcatg
      421 agagtatgtg agaaagtcga aagcaaaggg agaacaacct acaatgaggt ggcagacgaa
      481 ctggttgctg agttttcaga tcccaatatt aatattgact ctccagatcc tgataattcg
      541 tctgcgcaac aatatgatga gaaaaatatc cgaagaagag tttatgatgc gctgaatgtc
      601 ctaatggcta tggaaattat atctaaagat aaaaaggaaa tacagtggag aggtctgccc
      661 aagacaagta tgaatgatat tgaagaactg aagacagagg tcaatggact gaaaggtcgg
      721 attgacaaga aaaatgcata tctgcaggat ttacaagatc aatatgcatg tctgcaaaac
      781 ttggttcggc gaaatgagca gctatatggg tcaggagatg caccttccgg tggagtggcc
      841 ttgccattta tactagttca gacacgtcct catgcaactg tagaagtgga gatatcagaa
      901 gatatgcagc acgtgcattt tgatttcaat agcactccat ttgagttgca ggatgattca
      961 tttgtactga aagcaatggc attatctggc gaagaagctg attgtaccca agctcctggt
     1021 gcgaatggag gcgagtgctc aagtacacgg aacaattatt ggcaccacca ccaacaaccg
     1081 cagcctccag ggccaagagt agttagggta ccaaactcgc ctcctattcc agggatacta
     1141 aaagggcgtg tcaagcatga acacaagggt gggcgcgccg acccagcttt cttgtacaaa
     1201 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1261 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1321 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1381 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1441 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1501 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1561 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1621 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1681 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1741 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1801 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1861 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1921 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1981 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2041 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2101 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2161 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2221 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2281 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2341 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2521 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2761 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     2821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3001 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3061 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3121 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3181 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3241 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3301 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3361 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3421 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3481 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3541 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3601 acggccagtc ttaagctcgg gccc
//
