LOCUS       sequence-131235-        8447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema7A protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema7a-AP-His
FEATURES             Location/Qualifiers
     source          1..8447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5027..5044
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5070..5191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5107..5126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5161..5180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5229..5246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5400..5988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5480..5499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6159..6950,6951..7019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6782..6801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7020..7124)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7192..7210
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7248..7270)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7272..7292)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7277..7607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7458..7593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7520..7539
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7744..7763
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7830..8285)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7967..7988)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8179..8198
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8411..8433
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8425..8442
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8426..8442
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgacgcctcc
     1621 tcctcccgga cgtgccgccc ccagcgcacc gcgcgcccgc gtcctcagcc tgccggctcg
     1681 gttcgggctc ccgctgcggc tgcggcttct gctggtgttc tgggtggccg ccgcctccgc
     1741 ccaaggccac tcgaggagcg gaccccgcat ctccgccgtc tggaaagggc aggaccatgt
     1801 ggactttagc cagcctgagc cacacaccgt gcttttccat gagccgggca gcttctctgt
     1861 ctgggtgggt ggacgtggca aggtctacca cttcaacttc cccgagggca agaatgcctc
     1921 tgtgcgcacg gtgaacatcg gctccacaaa ggggtcctgt caggacaaac aggactgtgg
     1981 gaattacatc actcttctag aaaggcgggg taatgggctg ctggtctgtg gcaccaatgc
     2041 ccggaagccc agctgctgga acttggtgaa tgacagtgtg gtgatgtcac ttggtgagat
     2101 gaaaggctat gcccccttca gcccggatga gaactccctg gttctgtttg aaggagatga
     2161 agtgtactct accatccgga agcaggaata caacgggaag atccctcggt ttcgacgcat
     2221 tcggggcgag agtgaactgt acacaagtga tacagtcatg cagaacccac agttcatcaa
     2281 ggccaccatt gtgcaccaag accaagccta tgatgataag atctactact tcttccgaga
     2341 agacaaccct gacaagaacc ccgaggctcc tctcaatgtg tcccgagtag cccagttgtg
     2401 caggggggac cagggtggtg agagttcgtt gtctgtctcc aagtggaaca ccttcctgaa
     2461 agccatgttg gtctgcagcg atgcagccac caacaggaac ttcaatcggc tgcaagatgt
     2521 cttcctgctc cctgacccca gtggccagtg gagagatacc agggtctatg gcgttttctc
     2581 caacccctgg aactactcag ctgtctgcgt gtattcgctt ggtgacattg acagagtctt
     2641 ccgtacctca tcgctcaaag gctaccacat gggccttccc aaccctcgac ctggcatgtg
     2701 cctcccaaaa aagcagccca tacccacaga aaccttccag gtagctgata gtcacccaga
     2761 ggtggctcag agggtggaac ctatggggcc actgaagaca ccattgttcc attctaagta
     2821 ccattaccag aaagtggtcg ttcaccgcat gcaagccagc aatggagaga ccttccatgt
     2881 gctttatcta accacagaca ggggcaccat tcacaaggtg gtggaatcag gggaccagga
     2941 ccatagcttt gtcttcaata tcatggagat ccagcccttt caccgtgcag cggccatcca
     3001 ggctatatca ttggatgctg accggcggaa gctctatgtg acctcccagt gggaagtgag
     3061 ccaggtaccc ctggacatgt gtgaggtcta cagcgggggc tgccatggct gcctcatgtc
     3121 ccgagacccc tactgtggct gggaccagga ccgctgcgtg tctatctaca gctcccaacg
     3181 atcagtgctg cagtccatta atccagcgga gccacacaga gagtgtccca accctaaacc
     3241 agacgaggct ccattgcaga aggtttccct ggctcggaac tctcgatact acctgacctg
     3301 ccccatggag tcccgccacg ccacttactt atggcgccat gaggagaatg tggaacagag
     3361 ctgtgagcca ggccaccaaa gccctagctg catcctgttc attgagaacc tcacggcccg
     3421 tcagtatggc cactaccgct gcgaggccca agagggctcc tacctccgtg aggctcaaca
     3481 ctgggagctg ctgccagagg acagagcact ggccgaacaa ctaatgggcc atgcccgggg
     3541 cgcgcctgga tcttccggaa tcatcccagt tgaggaggag aacccggact tctggaaccg
     3601 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa
     3661 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat
     3721 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt
     3781 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc
     3841 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc
     3901 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa
     3961 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc
     4021 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt
     4081 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat
     4141 ggacattgac gtgatcctag gtgggggccg aaagtacatg tttcgcatgg gaaccccaga
     4201 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt
     4261 gcaggaatgg ctggcgaagc gccagggtgc ccggtacgtg tggaaccgca ctgagctcat
     4321 gcaggcttcc ctggacccgt ctgtggccca tctcatgggt ctctttgagc ctggagacat
     4381 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc
     4441 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg
     4501 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt
     4561 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt
     4621 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat
     4681 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg
     4741 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag
     4801 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg
     4861 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga
     4921 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg
     4981 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtcatc atcaccatca
     5041 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     5101 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5161 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct
     5221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7141 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7201 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7261 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     7321 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7381 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7561 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7621 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7681 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7741 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7801 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     7861 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7921 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7981 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8041 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8101 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8161 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8221 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8281 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8341 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8401 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
