LOCUS       sequence-13125-e        3594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1753-HQ858769
FEATURES             Location/Qualifiers
     source          1..3594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1151..1250)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1267..1286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1268..1286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1291..1307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1409..1428)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1420..2229
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1487..1506)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2376..2964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2865..2884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3118..3135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3294..3321)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3413..3499)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3548..3570
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3562..3579
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3563..3579
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggacgcc gccgtctcca ccctcaccgc tctcgccatc
      181 ttcgcgagca ccgtcgagca cgccgcctac aggagcgtgc aggggtatag ggtggtcggg
      241 aggaaaggcg gcgggtgggt ccgttgggag aggtgggtgg agcggcagtt cgtcctctcc
      301 ctctccttcc cacggtgcgt cgaggttgcc ctgcccgccg ccgcgccgcg gatactgcca
      361 gcggggtggc gcagccggcc ggtgttccgc gagggacaga ccgtcgacac ttggcgttgc
      421 attgtggcat tcgactccgt cgccgccgtc gctccgtcct ccccgccgcc gcccgtgctc
      481 tcccccttcg tgaacccaca gctgcagtat ctgtctaatc tgtacaatga cctgctgatg
      541 gtgtttcggt ttcgagaaga gaaaacggtc ccacaactcg ttaattcaaa gcaacccatc
      601 cgttgtggtg aacaggggaa aacttctgat ttgggtgatg catcggagtc cgattcggat
      661 ggggattgtc aatctggcaa agaacttgca ccaccagttc agaagcatac aagagccaac
      721 cggaagcata tagatagcat tactttagtt gatatagctc agtacttcca tcttccaatc
      781 cgagatgcat caaagacact caagattggg gtcagcatat tgaagaggaa atgccgacag
      841 tatgggatac ctcgttggcc tcaccggaag atcaagtcac ttgactctct cattcatgac
      901 cttgagttcg tgctagcaag agaggatgag gatgaggagg aggagaagca gctgcagaag
      961 gacaggctgg ctgctgcgat aaacgccctc acaaaacgga agagcatgct ggagagcgag
     1021 aaggaaacca tacagcagaa accggccatg gacctgatgg ctgaaaccaa gctattcagg
     1081 gaagatgttt tcaagaggag atatagggcc aaaagttcag tcatggatat ggataagggt
     1141 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1201 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1261 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1321 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1381 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1441 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1501 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1561 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1621 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1681 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1741 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1801 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1861 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1921 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1981 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2041 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2101 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2161 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2221 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2281 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2521 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2761 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3001 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3061 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3121 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3181 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3241 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3301 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3361 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3421 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3481 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3541 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
