LOCUS       sequence-131254-        7838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the entire Contactin 2, isoform 2 protein, C-terminally 
            fused to the Fc region of human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn4.2-Fc-His
FEATURES             Location/Qualifiers
     source          1..7838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4418..4435
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4461..4582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4498..4517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4552..4571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4620..4637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4791..5379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4871..4890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5550..6341,6342..6410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6173..6192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6411..6515)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6583..6601
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6639..6661)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6663..6683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6668..6998
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6849..6984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6911..6930
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7135..7154
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7221..7676)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7358..7379)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7570..7589
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7802..7824
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7816..7833
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7817..7833
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgaggttgcc
     1621 atgggaactg ctagtgctgc aatcattcat gttgtgcctt gcagatgact acacactgca
     1681 tggcccagtt tttgttcaag aaccaagtca tgtcatgttt cctttggatt ctgaggagaa
     1741 aaaagtgaaa ctcagttgtg aagttaaagg gaatccaaaa cctcacatca ggtggaagtt
     1801 aaatggaaca gatgttgaca ttggtatgga tttccgctac agtgttgttg acggcagctt
     1861 gttgatcaat aaccccaata aaacccaaga tgctggaacg taccagtgca tagcaacaaa
     1921 ctcatttgga acaattgtaa gcagagaagc aaagcttcag tttgcttatc ttgaaaactt
     1981 taagacaaga acaagaagca ctgtgtcagt ccgtcgaggt cagggaatgg tccttctgtg
     2041 cggcccgcca ccccattctg gagagctgag ctatgcctgg atcttcaatg aatacccttc
     2101 ctatcaggat aaccgccgct tcgtttctca agagactggg aatctgtata ttgccaaagt
     2161 ggaaaaatct gatgttggta attacacctg tgtagttacc aacactgtga ccaaccacaa
     2221 ggtcctggga ccgcccacac cactaatcct gagaaatgat ggggtgatgg gtgaatatga
     2281 gcccaaaata gaagtacagt ttccagaaac cgtcccggct gagaaaggaa caacagtcaa
     2341 actggaatgc tttgccttag gaaatccagt cccaactatt ctatggcgaa gagctgatgg
     2401 caagccaata gcaaggaaag ccagaagaca taagtcgaat ggaattctgg aaattcccaa
     2461 ttttcagcag gaagatgccg gttcatatga atgtgtggct gaaaactcca gaggcaaaaa
     2521 cgtagccaag ggacagttga ctttttatgc tcaacctaac tgggttcaaa taataaatga
     2581 tatccacgtg gccatggaag agagtgtctt ctgggaatgt aaagcaaatg ggaggcctaa
     2641 acctacctac agatggctaa aaaatggtga cccactgtta acacgggata gaatacaaat
     2701 cgagcaagga acactcaaca taacaatagt gaatctctca gatgctggca tgtaccagtg
     2761 tgtggcagaa aataaacatg gagttatctt ttccagcgct gagcttagtg taatagctga
     2821 aagtccagat ttttcccgaa cactcttaaa aagagtaaca cttgtcaaag tgggaggtga
     2881 agttgtcatt gagtgcaagc caaaagcatc cccaagacct gtctacacct ggagaaaggg
     2941 aagagaaata ttaagagaaa atgaaagaat taccatttct gaagatggga acctcagaat
     3001 catcaatgtg actaaatctg atgctggaag ttacacctgc atagccacga accattttgg
     3061 aactgccagc agtactggga atgtggttgt gaaagaccca acaaaggtga tggttcctcc
     3121 ttccagcatg gatgtcactg taggagaaag cattgttctc ccatgccagg taacacatga
     3181 tcactccctg gacattgtgt ttacgtggac atttaatgga cacttgatag actttgacaa
     3241 ggatggagac cactttgaaa gagttggagg gcaggattca gctggtgatt tgatgatccg
     3301 aaacatccaa ctgaagcatg ctgggaagta tgtctgcatg gtccaaacaa gtgtagacaa
     3361 actctctgtt gctgcggacc tgattgtaag aggtcctcca ggtcctcctg aggctgtgac
     3421 catagatgaa atcacagaca ccactgctca gctttcttgg agaccaggac ctgacaacca
     3481 cagccccatc accatgtatg ttatccaagc taggactcca ttctccgtgg gctggcaagc
     3541 agtcaataca gttccagatc tcgtcgacgg gaagacattc acagcaactg tggtgggttt
     3601 gaatccttgg gttgaatatg aattccgcac tgttgcagct aatgtgattg gcattgggga
     3661 acccagccgg ccctcagaga aacggaggac agaagaggct cagaacactg gggatcagag
     3721 aggtactagt gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg
     3781 accgtcagtc ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc
     3841 tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg
     3901 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa
     3961 cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa
     4021 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
     4081 caaagccaaa gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggatga
     4141 gctgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
     4201 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
     4261 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
     4321 gcagcagggg aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac
     4381 acagaagagc ctctccctgt ctccgggtaa aggcggccat catcaccatc accattaatc
     4441 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     4501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4561 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     4621 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4681 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5221 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5521 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5581 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5641 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5701 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5761 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5821 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5881 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5941 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6001 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6061 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6121 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6181 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6241 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6301 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6361 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6421 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6481 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6541 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6601 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6661 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     6721 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6781 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6841 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6901 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6961 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7021 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     7141 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     7201 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     7261 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7321 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7381 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7441 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7501 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7561 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7621 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     7681 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7741 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7801 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
