LOCUS       sequence-131260-        7340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the FLRT2 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Flrt2-Fc-His
FEATURES             Location/Qualifiers
     source          1..7340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3920..3937
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3963..4084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4000..4019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4054..4073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4122..4139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4293..4881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4373..4392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5052..5843,5844..5912))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5675..5694
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5913..6017)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6085..6103
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6141..6163)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6165..6185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6170..6500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6351..6486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6413..6432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6637..6656
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6723..7178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6860..6881)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7072..7091
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7304..7326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7318..7335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7319..7335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgggcctaca
     1621 gactacaaag tggcccggcc gtggggcttt catcctcaaa ttttggctca tcatttccct
     1681 gggactctac ttacaagtgt caaaactcct ggcctgccct agtgtatgcc gctgtgacag
     1741 gaactttgtc tactgtaacg agagaagctt gacctcagtg cctcttggga tcccggaggg
     1801 cgtaaccgta ctctacctcc acaacaacca aattaataat gctggctttc ctgcagagct
     1861 gcacaatgtc cagtcagtgc acactgtcta cctttatggc aaccagttgg atgagttccc
     1921 catgaacctt cccaagaatg tcagagttct ccatctgcag gaaaacaaca ttcagaccat
     1981 ctcgcgggct gctctcgctc agctcctcaa gctggaagaa ctccacctgg acgacaactc
     2041 catatctaca gtgggagtag aagacggagc gttccgggag gcgattagcc tcaaactgtt
     2101 gtttttatcg aagaatcacc tgagtagcgt gcctgttggg cttcctgtag acttgcaaga
     2161 gctgagagtg gatgaaaacc gaattgccgt catatcagac atggcctttc agaacctcac
     2221 aagcttggag cgcctgatcg tggatgggaa tcttctgacc aacaagggca ttgctgaggg
     2281 taccttcagt catctcacca agctcaagga attttctata gtccggaact cgctctccca
     2341 cccacctcct gatctcccag gtacgcatct gatcaggctc tacttgcagg ataaccagat
     2401 aaaccatatc ccattgacag ccttcgcaaa cctccgtaag ctggaaaggc tagatatatc
     2461 caacaaccag ctacgaatgt taactcaagg agtcttcgat catctctcca acctgaaaca
     2521 actcactgcg cggaataacc cttggttttg tgactgcagc attaaatggg tcacagaatg
     2581 gctcaagtat atcccttctt ctctcaacgt gcgtggtttc atgtgccaag gtcctgagca
     2641 agtccgcggg atggctgtca gggagttgaa tatgaatctt ttgtcttgtc ccaccacgac
     2701 tcctggccta cctgtcttta ccccagctcc aagtaccgtt tctccaacaa ctcagtctcc
     2761 tactctctct gttccaagcc ccagcagagg ctctgtgcct ccagctccta ccccatcgaa
     2821 acttcccacc atccctgatt gggatggcag agaaagagtg accccaccta tttctgaaag
     2881 gatccaactt tccatccact ttgttaatga tacttcgatt caagtcagct ggctgtctct
     2941 ctttactgtg atggcctaca aactgacatg ggtgaaaatg ggccacagtc tcgtaggggg
     3001 catcgttcag gaacgaattg tcagtggtga gaagcaacac ctgagcttgg ttaatttaga
     3061 gcccagatcc acgtatagga tttgtttagt gccgctggat gcgttcaact accgcactgt
     3121 ggaagatacc atctgttcgg aggctaccac ccatgcctct tatttgaaca acggcagcaa
     3181 cactgcttct agccatgagc agacgacttc ccacagtatg ggctccacta gtgacaaaac
     3241 tcacacatgc ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt
     3301 ccccccaaaa cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt
     3361 ggtggacgtg agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga
     3421 ggtgcataat gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt
     3481 cagcgtcctc accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt
     3541 ctccaacaaa gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc
     3601 ccgagaacca caggtgtata ccctgccccc atcccgggat gagctgacca agaaccaggt
     3661 cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag
     3721 caatgggcag ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc
     3781 cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt
     3841 ctcatgctcc gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct
     3901 gtctccgggt aaaggcggcc atcatcacca tcaccattaa tctagagatc ttattaaagc
     3961 agaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4081 cttatcatgt ctggtcgact ctagctagac tcttccgctt cctcgctcac tgactcgctg
     4141 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5041 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5101 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5161 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5221 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5281 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5341 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5401 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5461 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5521 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5581 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5641 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5701 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5761 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5821 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5881 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5941 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6001 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6061 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     6121 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggaggaatg tgtgtcagtt
     6181 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca
     6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc gtcccggaga
     6541 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6601 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6661 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6721 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6781 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6841 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6901 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6961 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     7021 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7081 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7141 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     7201 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     7261 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     7321 aaaacgacgg ccagtgaatt
//
