LOCUS       sequence-131263-        7497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Islr2.b protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Islr2.b-Fc-His
FEATURES             Location/Qualifiers
     source          1..7497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4076..4093
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4120..4241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4157..4176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4211..4230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4279..4296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4450..5038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4530..4549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5209..6000,6001..6069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5832..5851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6070..6174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6242..6260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6298..6320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6322..6342)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6327..6657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6508..6643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6570..6589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6794..6813
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6880..7335)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7017..7038)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7229..7248
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7461..7483
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7475..7492
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7476..7492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggggccctt
     1621 tggagccctg tgtttggcct gggctttgct aggagtggtc agagcgtgtc ccgagccttg
     1681 cgcctgtgtt gacaagtacg cccaccagtt tgcagactgt gcctacaagg agctgcgcga
     1741 ggtcccagaa ggactgccag ccaacgtgac cacgcttagt ctgtctgcca acaagattac
     1801 ggtactaagg cggggggcct tcgtcaacgt cacgcaggtc acttcgctgt ggctggctca
     1861 cagtgaggta cgcacggtag agtcaggggc attggcagtg ctgagtcagc tcaagaacct
     1921 cgacctaagc cacaacctta tatccaactt cccttggagc gaccttcgta acttgagcgc
     1981 gctgcagctg ctgaaaatga accacaaccg tctgggatcg ctgccccggg atgcactcgg
     2041 cgcgctcccg gacctgcgct ctctgcgcat caacaacaac cggctgcgta ccctggagcc
     2101 cggcacgttc gacgcactaa gcgcgctgtc tcacctgcaa ctctatcaca accccttcca
     2161 ctgcagctgt ggtctcgtgt ggctgcaggc ctgggcagcg agcacccggg tctccttacc
     2221 ggagcccgat tctatagcgt gcgcctcgcc ccctgagctg cagggcgtgc ccgtgcaccg
     2281 cctgcccgcc ctgccctgcg caccccccag cgtgcgtctg agcgcagagc cgccgcctga
     2341 ggcacctggc acccctctgc gcgcaggctt ggctttcacg ttacattgcg tcgccgaagg
     2401 ccaccccaca ccccgcctgc aatggcaact tcagatcccg ggtggcactg tagtcttagt
     2461 gccaccggtt ctcagcaagg aagaagatgg aggagataag gtggaggatg gagagggtga
     2521 cggagatgag gacctgccta cgcagactga agcgccaacc ccaactccag cacctgcttg
     2581 gccagcgcct ccagccaccc cgcgcttctt ggccctcgca aatggctctc tgttggtgcc
     2641 ccttctgagt gccaaggagg caggcatcta cacttgtcgt gcacacaatg agctgggcac
     2701 caactcaacg tccttacggg tgacggtggc tgcagcgggg ccgccaaaac acgctcctgg
     2761 aacaggggaa gaacctgatg cgcaggtccc gacctctgag cgcaaggcca ccactaaggg
     2821 ccgtagcaac agcgtcctgc ccttcaagcc tgaaggcaaa accaaaggcc aaggtcttgc
     2881 ccgagtcagc gtgcttgggg aaatcgaggc agagctggag gagacagatg aaggagagca
     2941 gatggaaggt cagatccctg cagatccgat gggagagaag cactgtggcc atggggaccc
     3001 ctctcgctat gtgtctaacc atgcattcaa ccagagctca gatctcaagc cgcacgtttt
     3061 tgagttgggc gtcatcgcgc tggatgtagc agagcgtgag gctcgggtgc agctgacgcc
     3121 ccttgctgcg cgctggggcc ctgggccaga tggtgctagc ggagcgcggc ggccggggag
     3181 gcggccactg cgcctactct atctgtgtcc tgcggggggt ggcacggcag ttcagtggtc
     3241 acgagtggaa gagggggtca atgcctactg gtttcgcggc ctgcggcctg gcaccaacta
     3301 ctccgtatgc ctggcactgg cgggcgaggc gtgtcacgtg caggtggtgt tttctaccaa
     3361 gaaagaactg ccgtccctgg gcgcgcctga caaaactcac acatgcccac cgtgcccagc
     3421 acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct
     3481 catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc
     3541 tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc
     3601 gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca
     3661 ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc
     3721 catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccct
     3781 gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg
     3841 cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta
     3901 caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac
     3961 cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc
     4021 tctgcacaac cactacacac agaagagcct ctccctgtct ccgggtaaag gcggccatca
     4081 tcaccatcac cattaatcta gaggatctta ttaaagcaga acttgtttat tgcagcttat
     4141 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4201 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta
     4261 gctagactct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6241 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6301 ctgacacatg cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6361 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6421 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6481 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6541 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6601 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6661 ctcccgggag cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg
     6721 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     6781 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     6841 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
     6901 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6961 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     7021 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7081 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     7141 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     7201 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     7261 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     7321 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     7381 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     7441 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
