LOCUS       sequence-13127-e        3294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1757-HQ858771
FEATURES             Location/Qualifiers
     source          1..3294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(851..950)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(967..986)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(968..986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(991..1007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(991..1007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1109..1128)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1120..1929
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1187..1206)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2076..2664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2565..2584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2818..2835
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2994..3021)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3113..3199)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3248..3270
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3262..3279
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3263..3279
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgcaggca tatatggagg gaggccagtt gttgggggct
      181 tgctgccttc ctggcttcct tgtgccggat cactacgccg ccttccctct tcctctcccg
      241 ctacaacttc ctagccagcc aaacgataat aggcttttcc agatgccgtt tgaccaggaa
      301 gaagccgaga accatggcgg gatgctctac tccgaccaat gtggactata cccgctgccg
      361 gccttcggca gctgctctgc tgccgccggc gcaacagcga agcctactgc tggtttcatg
      421 cccagtacta ttggcgctga gacgaaggtc tgcacctcgc tgactactaa aggttgcaac
      481 gagagtaata gcacatggtg gaaggggtca gcagctacga tagcggagag agggaagatg
      541 aaggtgagga ggaagatgag ggaaccgagg ttttgcttcc agaccagaag cgatgtggat
      601 gtactggatg atggctacaa gtggaggaag tacgggcaga aggttgtcaa gaacagcctc
      661 catccaagga gctatttccg gtgcacccac agcaactgcc gcgtgaagaa acgggtggag
      721 cggctgtcga cggactgccg catggtgatg accacgtacg agggccgcca cacgcactct
      781 ccctgcagcg acgacgcttc ctccggcgac cacaccgatt gcttcagctc ctttaagggt
      841 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      901 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      961 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1021 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1081 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1141 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1201 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1261 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1321 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1381 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1441 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1501 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1561 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1621 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1681 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1741 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1801 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1861 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1921 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     1981 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2221 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2461 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2821 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2881 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2941 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3001 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3061 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3121 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3181 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3241 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
