LOCUS       sequence-131272-        7824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the NCAM1, isoform 0.0.0 
            protein, C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Ncam1_0.0.0-Fc-His
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1648..1659
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4404..4421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4447..4568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4484..4503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4538..4557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4606..4623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4777..5365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4857..4876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5536..6327,6328..6396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6159..6178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6397..6501)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6569..6587
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6625..6647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6649..6669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6654..6984
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6835..6970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6897..6916
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7121..7140
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7207..7662)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7344..7365)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7556..7575
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7788..7810
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7802..7819
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7803..7819
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgccaccatg ctgcgaacta
     1621 aggatctcat ctggactttg tttttcctgg gaactgcagt ttccctgcag gtagatattg
     1681 ttcccagcca aggagaaatc agcgttggag agtccaaatt cttcctgtgt caagtggcag
     1741 gagatgccaa agataaggac atctcctggt tctcccccaa tggggagaag ctgagcccaa
     1801 accagcagcg gatctcagtg gtgtggaatg atgacgactc ctctaccctc accatctaca
     1861 acgccaacat cgacgatgcc ggcatataca agtgtgtggt cacggctgag gacggcaccc
     1921 agtctgaggc cactgtcaac gtgaagatct tccagaagct catgttcaag aatgcaccaa
     1981 ccccacagga gtttaaggaa ggggaggatg ctgtgattgt ctgtgatgtg gtcagctccc
     2041 tgcctccaac catcatctgg aaacacaaag gccgagatgt cattctgaaa aaagacgtcc
     2101 ggttcatagt cctgtccaac aactacctgc agatcagggg catcaagaaa acagatgagg
     2161 gtacttaccg ctgtgagggc aggatcctgg cccgcgggga aatcaacttc aaggacattc
     2221 aggtcattgt gaatgtacca cccactgtcc aggccagaca gagcatcgtg aatgccactg
     2281 ccaacctggg ccagtctgtc accctggtgt gtgatgccga tggcttccca gagcccacca
     2341 tgagctggac aaaggatggg gaacccatag agaacgagga ggaagatgac gagaagcaca
     2401 tcttcagtga tgacagctcc gagctgacca tcaggaatgt ggataaaaac gacgaggccg
     2461 aatacgtctg catcgcagag aacaaggctg gcgagcagga tgcctccatc cacctcaagg
     2521 tctttgcaaa gcccaaaatc acctatgtag agaatcagac ggccatggaa ctagaggagc
     2581 aagtcactct gacctgtgaa gcctccggag accccatccc ctccatcacc tggagaacat
     2641 ccacccggaa catcagcagt gaagaaaaga ctctggatgg gcacatggtg gttcgcagcc
     2701 atgctcgtgt gtcctccttg accctgaaga gcatccagta cacagatgct ggagaataca
     2761 tctgcactgc cagcaacacc atcggccagg attcccagtc catgtacctc gaagttcaat
     2821 atgctcccaa gctccagggc cctgtggctg tgtacacttg ggaagggaac caggtgaaca
     2881 tcacctgtga ggtcttcgcc tacccaagtg ccacaatctc ctggttccga gatggtcagt
     2941 tgctgccaag ctccaactac agcaatatca agatctacaa caccccatct gctagctatc
     3001 tggaggtgac ccctgattca gaaaatgact ttggaaacta caactgtaca gcagtgaacc
     3061 gtattggaca ggagtccttg gaattcatcc ttgttcaagc agacacaccg tcttctccat
     3121 ccattgaccg ggtggaacca tactccagca cagcgcaggt gcagtttgat gagccagagg
     3181 ccacaggggg cgtgcccatt ctcaagtaca aggctgagtg gaagtcgctg ggcgaagaat
     3241 cctggcattt caagtggtat gatgccaaag aagccaacat ggaaggcatt gtcaccatca
     3301 tgggcctgaa acctgagacg aggtactcgg tacgactggc ggccctcaac ggcaaggggc
     3361 tgggcgagat cagtgcagcc actgagttca agacacagcc agtccgggaa cccagtgcac
     3421 ccaagctgga agggcagatg ggagaggacg ggaactccat caaggtgaac ctgatcaagc
     3481 aggatgacgg aggctccccc atcagacact atctggtcaa gtacagagcg ctcgcctctg
     3541 agtggaaacc ggaaatcagg ctcccatccg gcagtgacca cgtcatgctc aagtccctgg
     3601 actggaacgc agagtatgaa gtctatgtgg tagctgaaaa ccagcaagga aaatccaagg
     3661 cagctcactt tgtgttcagg acctcagccc agcccacggc catcccagcc actagtgaca
     3721 aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc
     3781 tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg
     3841 tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg
     3901 tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg
     3961 tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca
     4021 aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc
     4081 agccccgaga accacaggtg tataccctgc ccccatcccg ggatgagctg accaagaacc
     4141 aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg
     4201 agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg
     4261 gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg
     4321 tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag aagagcctct
     4381 ccctgtctcc gggtaaaggc ggccatcatc accatcacca ttaatctaga gatcttatta
     4441 aagcagaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4501 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4561 gtatcttatc atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc
     4621 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5461 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5521 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5581 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5641 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5701 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5761 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5821 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5881 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5941 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6001 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6061 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6481 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     6541 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     6601 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc
     6661 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat
     6721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6901 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6961 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg
     7021 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     7081 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     7141 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7201 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     7261 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7321 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7381 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7441 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     7501 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     7561 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     7621 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     7681 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7741 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     7801 ttgtaaaacg acggccagtg aatt
//
