LOCUS       sequence-131275-        9122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neogenin 1, isoform a 
            protein, C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Neo1.a-Fc-His
FEATURES             Location/Qualifiers
     source          1..9122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5702..5719
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5745..5866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5782..5801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5836..5855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5904..5921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6075..6663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6155..6174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6834..7625,7626..7694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7457..7476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7695..7799)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7867..7885
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7923..7945)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7947..7967)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7952..8282
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8133..8268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8195..8214
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8419..8438
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8505..8960)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8642..8663)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8854..8873
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9086..9108
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9100..9117
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9101..9117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcggcgga
     1621 gcgcgaagcc gggcgactcc tctgcacctc ctcctcccgg cgctgctgtc cgccaccgcc
     1681 gctgctgctg ttgctgccgc tgctgctgct gctcggacgc ccggcgtccg gcgccgcggc
     1741 cacgaagagc ggctccccgc cgcagtccgc aggagccagt gttcgaacat tcactccgtt
     1801 ttattttctg gtggagccag tagacaccct ctcagttaga ggctcttctg ttatattaaa
     1861 ttgctcggca tattctgagc cctctccaaa cattgaatgg aagaaagatg ggactttttt
     1921 aaacttagaa tcagatgatc gacgccagct actcccagat ggatctttat tcatcagcaa
     1981 cgtggtgcat tccaaacaca ataagcctga cgaaggtttc tatcagtgtg tagccactgt
     2041 ggataatctt ggaaccattg tcagcagaac agccaagctc acagtagcag gtcttccaag
     2101 atttaccagc caaccagaac cttcttcagt ctatgttgga aacagtgcaa ttctgaattg
     2161 tgaagttaat gcagatttgg tcccatttgt taggtgggaa cagaatcgac agccccttct
     2221 tctagatgac aggattgtca aacttccaag tggaacactg gttatcagca atgctactga
     2281 aggagatggg ggactctacc gctgcattgt tgaaagtggt gggccaccaa agtttagtga
     2341 cgaagctgaa ttgaaagttc ttcaagatcc tgaggaaatt gtagacttgg tatttctgat
     2401 gcgaccatct tctatgatga aagtcactgg tcagagtgca gtgttgccat gtgttgtctc
     2461 agggcttcct gctccagttg ttagatggat gaaaaacgaa gaagtgcttg acacagaaag
     2521 ctctggcagg ttggtcttgc tagcaggagg ttgcttggag atcagtgatg tcactgagga
     2581 tgatgctggg acttattttt gcatagctga taatggaaat aagacagttg aagctcaggc
     2641 ggagcttact gtgcaagtgc cacctggatt cctgaaacaa cctgctaaca tatatgctca
     2701 cgaatccatg gacattgtat ttgaatgtga agtcactggg aagccaactc caactgtgaa
     2761 gtgggtcaag aatggggatg tggttatccc cagtgattac tttaaaattg taaaggaaca
     2821 taatcttcaa gttttgggtc tggtgaaatc agatgaaggg ttctatcaat gcattgctga
     2881 gaatgatgtt ggaaatgcac aagctggagc ccagctgata atccttgagc atgatgttgc
     2941 catcccaaca ttacctccca cttcactgac cagtgccact actgaccatc tagcaccagc
     3001 cacaacggga ccattacctt cagctcctcg agacgtcgtg gcctccctgg tctctactcg
     3061 cttcattaaa ttgacatggc gtacacctgc atcagaccct catggagaca atctcaccta
     3121 ctctgtgttc tacaccaagg aaggggttgc tagggagcgt gttgagaata ccagccagcc
     3181 aggagagatg caggtgacta ttcaaaactt gatgccagca actgtgtaca tcttcaaagt
     3241 tatggctcaa aataagcatg gctctggaga aagttcagct cctcttcgag tagagacaca
     3301 gcctgaggtt cagctccctg gcccagcacc taatatccgt gcttatgcaa cgtcacctac
     3361 ttctatcact gtcacctggg aaacaccgtt atctggcaat ggggaaattc aaaattacaa
     3421 attgtactac atggaaaaag gaactgataa agaacaggat attgatgttt caagtcactc
     3481 ctacaccatt aatggactga agaaatacac agaatacagt ttccgagtgg tggcctacaa
     3541 taaacatggt cctggagttt ctacacaaga tgttgctgtt cgaacattat cagatgttcc
     3601 cagtgctgct cctcagaatc tgtccttaga agtgagaaat tcaaagagta tagtgatcca
     3661 ctggcagccc ccttcctcaa ccacacaaaa tgggcagata actggctaca agattcgata
     3721 tcgaaaggcc tcccgaaaaa gtgatgtcac tgagaccttg gtaactggga cacagctgtc
     3781 tcagctgatt gaaggtcttg atcgggggac agaatataac ttccgagtcg ctgctctcac
     3841 agtcaatggt acaggtccag caactgattg gctgtctgct gaaacttttg aaagcgacct
     3901 agatgaaact cgtgttcctg aagtgcccag ctctcttcat gtccgtccgc tcgtcactag
     3961 cattgtagtg agctggactc ctccagagaa ccagaacatt gtggtccgag gttatgccat
     4021 cggttacggc attggcagcc ctcatgccca gaccatcaaa gtggactata aacaacgtta
     4081 ttacaccatc gaaaacttgg atccaagctc tcattacgtg attaccttga aagcatttaa
     4141 caatgttggc gaaggcatcc ccctttatga gagtgctgtg accagacctc acacagacac
     4201 ttctgaagtt gatttatttg ttattaatgc tccatacact ccagtgccag atcccactcc
     4261 catgatgcca ccagtgggag ttcaggcttc cattctgagt cacgacacca taaggattac
     4321 ctgggcagac aactccctgc ccaaacacca gaagattaca gactcccgct actacacagt
     4381 ccggtggaag accaacatcc cagcaaacac gaagtacaag aatgcaaatg caacgacgtt
     4441 aagctatttg gttactggtt taaagccaaa tacgctctat gagttctctg tgatggtgac
     4501 caaaggcaga aggtcaagca cgtggagtat gacagctcat ggcgctacct ttgaattagt
     4561 tcctacttct ccacctaagg atgtgacagt tgtgagtaag gaaggaaaac ctagaaccat
     4621 catagtgaac tggcagcctc cctctgaagc taacggcaag attacaggtt acatcatcta
     4681 ttacagcacg gatgtgaatg cagagataca tgactgggtt attgaaccag ttgtgggaaa
     4741 cagactgact caccagattc aagagttaac acttgatacg ccatactact tcaaaatcca
     4801 ggcccggaac tcaaagggca tggggcccat gtctgaagct gtacagttca gaacacctaa
     4861 agcggactcc tctgataaaa tgcctaatga ccaagcctta gggtcagcag gaaaaggaag
     4921 ccgactacca gacctgggat ctgactacaa acctccaatg agtggcagca acagccctca
     4981 cgggagcccc acctcccctc tggacagcac tagtgacaaa actcacacat gcccaccgtg
     5041 cccagcacct gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga
     5101 caccctcatg atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga
     5161 agaccctgag gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac
     5221 aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct
     5281 gcaccaggac tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc
     5341 agcccccatc gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta
     5401 taccctgccc ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt
     5461 caaaggcttc tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa
     5521 caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa
     5581 gctcaccgtg gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca
     5641 tgaggctctg cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaaggcgg
     5701 ccatcatcac catcaccatt aatctagaga tcttattaaa gcagaacttg tttattgcag
     5761 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5821 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga
     5881 ctctagctag actcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5941 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6001 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6061 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6121 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6181 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6241 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6301 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6361 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6421 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6481 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6541 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6601 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6661 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6721 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6781 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7681 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7741 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7801 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7861 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7921 aacctctgac acatgcagct cccggaggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7981 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8041 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8101 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8161 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8221 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8281 aaaagctccc gggagcttgt atatccattt tcgtcccgga gacggtcaca gcttgtctgt
     8341 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     8401 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     8461 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     8521 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8581 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8641 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8701 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     8761 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8821 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     8881 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     8941 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9001 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9061 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     9121 tt
//
