LOCUS       sequence-131280-        9029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neogenin 1, isoform f 
            protein, C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Neo1.f-Fc-His
FEATURES             Location/Qualifiers
     source          1..9029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5609..5626
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5652..5773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5689..5708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5743..5762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5811..5828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5982..6570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6062..6081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6741..7532,7533..7601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7364..7383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7602..7706)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7774..7792
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7830..7852)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7854..7874)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7859..8189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8040..8175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8102..8121
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8326..8345
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8412..8867)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8549..8570)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8761..8780
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8993..9015
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9007..9024
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9008..9024
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcggcgga
     1621 gcgcgaagcc gggcgactcc tctgcacctc ctcctcccgg cgctgctgtc cgccaccgcc
     1681 gctgctgctg ttgctgccgc tgctgctgct gctcggacgc ccggcgtccg gcgccgcggc
     1741 cacgaagagc ggctccccgc cgcagtccgc aggagccagt gttcgaacat tcactccgtt
     1801 ttattttctg gtggagccag tagacaccct ctcagttaga ggctcttctg ttatattaaa
     1861 ttgctcggca tattctgagc cctctccaaa cattgaatgg aagaaagatg ggactttttt
     1921 aaacttagaa tcagatgatc gacgccagct actcccagat ggatctttat tcatcagcaa
     1981 cgtggtgcat tccaaacaca ataagcctga cgaaggtttc tatcagtgtg tagccactgt
     2041 ggataatctt ggaaccattg tcagcagaac agccaagctc acagtagcag gtcttccaag
     2101 atttaccagc caaccagaac cttcttcagt ctatgttgga aacagtgcaa ttctgaattg
     2161 tgaagttaat gcagatttgg tcccatttgt taggtgggaa cagaatcgac agccccttct
     2221 tctagatgac aggattgtca aacttccaag tggaacactg gttatcagca atgctactga
     2281 aggagatggg ggactctacc gctgcattgt tgaaagtggt gggccaccaa agtttagtga
     2341 cgaagctgaa ttgaaagttc ttcaagatcc tgaggaaatt gtagacttgg tatttctgat
     2401 gcgaccatct tctatgatga aagtcactgg tcagagtgca gtgttgccat gtgttgtctc
     2461 agggcttcct gctccagttg ttagatggat gaaaaacgaa gaagtgcttg acacagaaag
     2521 ctctggcagg ttggtcttgc tagcaggagg ttgcttggag atcagtgatg tcactgagga
     2581 tgatgctggg acttattttt gcatagctga taatggaaat aagacagttg aagctcaggc
     2641 ggagcttact gtgcaagtgc cacctggatt cctgaaacaa cctgctaaca tatatgctca
     2701 cgaatccatg gacattgtat ttgaatgtga agtcactggg aagccaactc caactgtgaa
     2761 gtgggtcaag aatggggatg tggttatccc cagtgattac tttaaaattg taaaggaaca
     2821 taatcttcaa gttttgggtc tggtgaaatc agatgaaggg ttctatcaat gcattgctga
     2881 gaatgatgtt ggaaatgcac aagctggagc ccagctgata atccttgagc atgcaccagc
     2941 cacaacggga ccattacctt cagctcctcg agacgtcgtg gcctccctgg tctctactcg
     3001 cttcattaaa ttgacatggc gtacacctgc atcagaccct catggagaca atctcaccta
     3061 ctctgtgttc tacaccaagg aaggggttgc tagggagcgt gttgagaata ccagccagcc
     3121 aggagagatg caggtgacta ttcaaaactt gatgccagca actgtgtaca tcttcaaagt
     3181 tatggctcaa aataagcatg gctctggaga aagttcagct cctcttcgag tagagacaca
     3241 gcctgaggtt cagctccctg gcccagcacc taatatccgt gcttatgcaa cgtcacctac
     3301 ttctatcact gtcacctggg aaacaccgtt atctggcaat ggggaaattc aaaattacaa
     3361 attgtactac atggaaaaag gaactgataa agaacaggat attgatgttt caagtcactc
     3421 ctacaccatt aatggactga agaaatacac agaatacagt ttccgagtgg tggcctacaa
     3481 taaacatggt cctggagttt ctacacaaga tgttgctgtt cgaacattat cagatgttcc
     3541 cagtgctgct cctcagaatc tgtccttaga agtgagaaat tcaaagagta tagtgatcca
     3601 ctggcagccc ccttcctcaa ccacacaaaa tgggcagata actggctaca agattcgata
     3661 tcgaaaggcc tcccgaaaaa gtgatgtcac tgagaccttg gtaactggga cacagctgtc
     3721 tcagctgatt gaaggtcttg atcgggggac agaatataac ttccgagtcg ctgctctcac
     3781 agtcaatggt acaggtccag caactgattg gctgtctgct gaaacttttg aaagcgacct
     3841 agatgaaact cgtgttcctg aagtgcccag ctctcttcat gtccgtccgc tcgtcactag
     3901 cattgtagtg agctggactc ctccagagaa ccagaacatt gtggtccgag gttatgccat
     3961 cggttacggc attggcagcc ctcatgccca gaccatcaaa gtggactata aacaacgtta
     4021 ttacaccatc gaaaacttgg atccaagctc tcattacgtg attaccttga aagcatttaa
     4081 caatgttggc gaaggcatcc ccctttatga gagtgctgtg accagacctc acacagacac
     4141 ttctgaagtt gatttatttg ttattaatgc tccatacact ccagtgccag atcccactcc
     4201 catgatgcca ccagtgggag ttcaggcttc cattctgagt cacgacacca taaggattac
     4261 ctgggcagac aactccctgc ccaaacacca gaagattaca gactcccgct actacacagt
     4321 ccggtggaag accaacatcc cagcaaacac gaagtacaag aatgcaaatg caacgacgtt
     4381 aagctatttg gttactggtt taaagccaaa tacgctctat gagttctctg tgatggtgac
     4441 caaaggcaga aggtcaagca cgtggagtat gacagctcat ggcgctacct ttgaattagt
     4501 tcctacttct ccacctaagg atgtgacagt tgtgagtaag gaaggaaaac ctagaaccat
     4561 catagtgaat tggcagcctc cctctgaagc taacggcaag attacaggtt acatcatcta
     4621 ttacagcacg gatgtgaatg cagagataca tgactgggtt attgaaccag ttgtgggaaa
     4681 cagactgact caccagattc aagagttaac acttgatacg ccatactact tcaaaatcca
     4741 ggcccggaac tcaaagggca tggggcccat gtctgaagct gtacagttca gaacacctaa
     4801 agccttaggg tcagcaggaa aaggaagccg actaccagac ctgggatctg actacaaacc
     4861 tccaatgagt ggcagcaaca gccctcacgg gagccccacc tcccctctgg acagcactag
     4921 tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt
     4981 cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac
     5041 atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga
     5101 cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta
     5161 ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa
     5221 gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa
     5281 agggcagccc cgagaaccac aggtgtatac cctgccccca tcccgggatg agctgaccaa
     5341 gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga
     5401 gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc
     5461 cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg
     5521 gaacgtcttc tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag
     5581 cctctccctg tctccgggta aaggcggcca tcatcaccat caccattaat ctagagatct
     5641 tattaaagca gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5701 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5761 tcaatgtatc ttatcatgtc tggtcgactc tagctagact cttccgcttc ctcgctcact
     5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     7741 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     7801 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaggaatgt
     7861 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca
     7921 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7981 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8041 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8101 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8161 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8221 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     8281 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     8341 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     8401 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     8461 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     8521 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     8581 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     8641 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     8701 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     8761 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     8821 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     8881 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     8941 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     9001 cgacgttgta aaacgacggc cagtgaatt
//
