LOCUS       sequence-131285-        8313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neuropilin 2, isoform 2 
            protein, C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Nrp2.2-Fc-His
FEATURES             Location/Qualifiers
     source          1..8313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4892..4909
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4936..5057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4973..4992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5027..5046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5095..5112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5266..5854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5346..5365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6025..6816,6817..6885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6648..6667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6886..6990)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7058..7076
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7114..7136)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7138..7158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7143..7473
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7324..7459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7386..7405
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7610..7629
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7696..8151)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7833..7854)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8045..8064
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8277..8299
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8291..8308
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8292..8308
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggatatgtt
     1621 tcctcttacc tgggttttct tagctctgta cttttcagga cacgaagtga gaagccagca
     1681 agatccaccc tgcggaggtc ggctgaattc caaagatgct ggctacatca cttccccagg
     1741 ctacccccag gactatccct cccaccagaa ctgtgagtgg attgtctacg cccccgaacc
     1801 caaccagaag attgttctca acttcaaccc tcactttgaa atcgagaaac acgactgcaa
     1861 gtatgacttc attgagattc gggatgggga cagtgagtca gctgacctcc tgggcaagca
     1921 ctgtgggaac atcgccccgc ccaccatcat ctcctcaggc tccgtgttat acatcaagtt
     1981 cacctcagac tacgcccggc agggggcagg tttctctcta cgctatgaga tcttcaaaac
     2041 aggctctgaa gattgttcca agaactttac aagccccaat gggaccattg aatctccagg
     2101 gtttccagag aagtatccac acaatctgga ctgtaccttc accatcctgg ccaaacccag
     2161 gatggagatc atcctacagt tcctgacctt tgacctggag catgaccctc tacaagtggg
     2221 ggaaggagac tgtaaatatg actggctgga catctgggat ggcattccac atgttggacc
     2281 tctgattggc aagtactgtg ggacgaaaac accctccaaa ctccgctcgt ccacggggat
     2341 cctctccttg acctttcaca cggacatggc agtggccaag gatggcttct ccgcacgtta
     2401 ctatttgatc caccaggagc cacctgagaa ttttcagtgc aatgtccctt tgggaatgga
     2461 gtctggccgg attgctaatg aacagatcag tgcctcctcc accttctctg atgggaggtg
     2521 gactcctcaa cagagccggc tccatggtga tgacaatggc tggacaccca atttggattc
     2581 caacaaggag tatctccagg tggacctgcg cttcctaacc atgctcacag ccattgcaac
     2641 acagggagcc atttccaggg aaacccagaa aggctactac gtcaaatcgt acaagctgga
     2701 agtcagcaca aatggtgaag attggatggt ctaccggcat ggcaaaaacc acaagatatt
     2761 ccaagcgaac aatgatgcga ccgaggtggt gctaaacaag ctccacatgc cactgctgac
     2821 tcggttcatc aggatccgcc cgcagacgtg gcatttgggc attgcccttc gcctggagct
     2881 ctttggctgc cgggtcacag atgcaccctg ctccaacatg ctggggatgc tctcgggcct
     2941 cattgctgat acccagatct ctgcctcctc cacccgagag tacctctgga gccccagtgc
     3001 tgcccgcctg gttagtagcc gctctggctg gtttcctcgg aaccctcaag cccagccagg
     3061 tgaagaatgg cttcaggtag acctggggac acccaagaca gtgaaagggg tcatcatcca
     3121 gggagcccga ggaggagaca gcatcactgc cgtggaagcc agggcgtttg tacgcaagtt
     3181 caaagtctcc tacagcctaa atggcaagga ctgggaatat atccaggacc ccaggactca
     3241 gcagacaaag ctgtttgaag ggaacatgca ctatgacacc cctgacatcc gaaggttcga
     3301 tcctgttcca gcgcagtatg tgcgggtgta cccagagagg tggtcgccag caggcatcgg
     3361 gatgaggctg gaggtgctgg gctgtgactg gacagactca aagcccacag tggagacgct
     3421 gggacccacc gtgaagagtg aagagactac caccccatat cccatggatg aggatgccac
     3481 cgagtgtggg gaaaactgca gctttgagga tgacaaagat ttgcaacttc cttcaggatt
     3541 caactgcaac tttgattttc cggaagagac ctgtggttgg gtgtacgacc atgccaagtg
     3601 gctccggagc acgtggatca gcagcgctaa ccccaatgac agaacatttc cagatgacaa
     3661 gaacttcttg aaactgcaga gtgatggccg acgagagggc cagtacgggc ggctcatcag
     3721 cccaccggtg cacctgcccc gaagccctgt gtgcatggag ttccagtacc aagccatggg
     3781 cggccacggg gtggcactgc aggtggttcg ggaagccagc caggaaagca aactcctttg
     3841 ggtcatccgt gaggaccagg gcagcgagtg gaaacacggg cgcattatcc tgcccagcta
     3901 tgacatggag tatcagatcg tgttcgaggg agtgataggg aagggacgat cgggagagat
     3961 ttccatcgat gacattcgga taagcactga tgtcccactg gagaactgca tggaacccat
     4021 atcagctttt gcagtggaca tcccagaaac ccatggggga gagggctatg aagatgagat
     4081 tgatgatgaa tatgaaggag attggagcaa ctcttcttcc tctacctcag gggctggtga
     4141 cccctcatct ggcaaagaaa agagctggct gtacacccta gatcccattc tgatcggcgc
     4201 gcctgacaaa actcacacat gcccaccgtg cccagcacct gaactcctgg ggggaccgtc
     4261 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
     4321 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
     4381 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac
     4441 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta
     4501 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
     4561 caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac
     4621 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
     4681 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
     4741 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca
     4801 ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacacagaa
     4861 gagcctctcc ctgtctccgg gtaaaggcgg ccatcatcac catcaccatt aatctagagg
     4921 atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5041 ctcatcaatg tatcttatca tgtctggtcg actctagcta gactcttccg cttcctcgct
     5101 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5161 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5221 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5281 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5341 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5401 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5461 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5521 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5581 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5641 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5701 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5761 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5821 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5881 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5941 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6001 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6061 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6121 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6181 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6241 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6301 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6361 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6421 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6481 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6541 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6601 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6661 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6721 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6781 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6841 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6901 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6961 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7021 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7081 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagga
     7141 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa
     7201 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7261 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7321 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7381 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     7441 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     7501 ttcgtcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7561 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7621 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7681 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     7741 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7801 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7861 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     7921 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     7981 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     8041 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     8101 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     8161 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8221 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     8281 gtcacgacgt tgtaaaacga cggccagtga att
//
