LOCUS       sequence-131312-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the entire extracellular region of the PlexinB1 protein 
            (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb1(L)-Fc-His
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      4038..4047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6722..6739
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6766..6887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6803..6822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6857..6876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6925..6942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7096..7684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7176..7195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7855..8646,8647..8715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8478..8497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8716..8820)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8888..8906
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8944..8966)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8968..8988)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8973..9303
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9154..9289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9216..9235
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9440..9459
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9526..9981)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9663..9684)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9875..9894
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10107..10129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10121..10138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10122..10138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtctgtcct
     1621 cggcccagtt cttctccagg tgttctgggc cgggtgtgtc gtcaccctgc ggtcccctct
     1681 gccagctgct ttcactgcca atggcacaca tctacaacac ttggcaaggg accccaccac
     1741 aggtaccctc tatgtagggg ccaccaactt cctgttccag ttgagccctg ggctgcagct
     1801 ggaagccgtg gtgtccacgg gccctgtgaa tgacagccgg gattgcctgc cacctgtgat
     1861 acctgatgaa tgtccccaag cccagcctac taacaaccct aaccagctgc tcctggtgag
     1921 cccagaggct ctggtggtgt gtgggagcat acaccagggc atctgtgagc tacggagcct
     1981 gggacagatc aggcagctgc tgctacggcc agagcgacct ggggacaccc agtatgtggc
     2041 tgcaaatgac cctgcagtca gtacagtggg gctggtggcc cagggattgg taggggagcc
     2101 cctcctgttt gtggggcggg gatacaccag caggggtgta ggtggtggga ttcctcccat
     2161 tacaacccga gccctgcgac caccggaccc ccaagctgcc ttctcttatg aagaaacagc
     2221 caagttagca gtgggccgcc tgtccgagta cagccaccac ttcgtgagtg cctttgtacg
     2281 cggggccagt gcatacttcc tgttcttgcg gcgagacctg aaggcccctt ctagagcttt
     2341 ccgtgcctat gtgtctcgag tgtgccttca ggaccagcac tactactctt atgtggaatt
     2401 gcccctggcc tgccagggtg gtcgttacgg tcttatccag gctgcagctg tagccacgtc
     2461 caaggaggtg gcccgtgggg acgtactctt tgcagctttc tcctcagtgg ctcctcccac
     2521 tgtggattgg cccctgtcag catctactgg ggcatctgga acctctgtgc tctgtgcctt
     2581 ccccctggat gaggtagacc agcttgctaa ttacactcga gatgcctgtt atactcggga
     2641 aggccgtgct gagaacggga ccaaggttgc tgacattgca tacgatgtcc tttccgactg
     2701 tgcgcagcta ccagtggaca ccccggatgc ttttccatgt ggctctgacc acacacccag
     2761 tcccatggtc agctgtgtcc ctttggaagc cacgccaatt ctggagctac caggggttca
     2821 gctaacagct gtggctgtca ccatggagga tggacacact attgctttcc tgggtgacag
     2881 tcaaggacag ttgcataggg tctacttagg ccctggaaga agtgctgccc catattctaa
     2941 acagagcatc cagccggggt ctcctgtgaa cagagatctt acctttgatg gtacctttga
     3001 gcatctctat gtagcaactc agactactct tgtgaaggtt cctgtggctc cttgtgctca
     3061 gcatctggac tgtgactctt gccttgctca cagggaccct tattgcggat ggtgtgtgct
     3121 cctgggcagg tgtagtcgcc ggtcggagtg ctcaagggac cagggcccag agcagtggct
     3181 gtggagcttc cagccggaac tgggctgtct tcgagtggtg gccgtgagcc ctgccaatat
     3241 cagtcgggaa gagaggaggg aggttttctt gtcagtgcca ggcctgccat ctctctggcc
     3301 aggggagtca tatttctgct actttggaga ccaacagagt cctgctctac tgaccagttc
     3361 tggtgtgatg tgtccctccc cagaccccag tgaggctcca gtgctgcaga gaggagccga
     3421 ccatatctct gtgaacgtgg agctcaggtt tggtgccgtg gtgatcgcca gcacctccct
     3481 ctccttctat gactgcgtgg cagttactgc gtcttcccca tctgcaccgt gccgggcctg
     3541 tgtgagcagc cgctggggct gtaactggtg tgtgtggcag cagctgtgca cacacaaggc
     3601 ctcgtgtgac gctgggccta tggtggcaag ccaacagagc ccactccttc ccctaatccc
     3661 tcctgcaagg gatgaactca cccctttccc acccacagtc ccccaaacca cggtcactcc
     3721 tacccccaac agcttcccaa tagagcctag ggctccctcc acagcctcag atgtcctacc
     3781 tggggccaag ccttcccggc tcagcctctg gggcccatgg gcaggtcctg gccccatact
     3841 ttcccctact tccacagagt cacctcttca tgagaagccc cttcctcctg acccccctac
     3901 catacctgga accactgtcc ctgcccccac tggcttggga ccatcgacca cacctgagga
     3961 cctcttggcc tcctacccat tcccctcaga tgcagctgca gtgtcccctg cagagcctgg
     4021 ccctgaggct ctgccttcca tggtggctct ggaccagccc cctggcactg ttccagacac
     4081 tactttccca ggggcccctg gctccatgaa gcccgttctg gattggctca ccaaaggagg
     4141 cggcgagctg cccgaggcgg atgagtggat ggggggtgac acgcccgcct tctccacttc
     4201 cacactcctc tcaggtgatg gagactcagc agagcacgag ggccctcctg cccccctcat
     4261 cctcctgtcc agcctcgact accagtacga cacccccggg ctctgggagc tgggagaggt
     4321 gaatcagagg gtgagctcct gcccctgtgt ggagaccgtc cagggctcct tgctgatacc
     4381 ggtccatgtg gaacgcgaag tccagcttcg aggcaggaac ctgtggcttt tccaggatgg
     4441 cccgaggagc agcgagtgtg tgctggagct agggagtcgg gaggtggctg tggaggctca
     4501 ggtggagtgt gcgccgcctc cagatgtctg gtgccacatc aagtgccagc agcatcagtt
     4561 cagctatgaa gctttgaagc cagaactgca ggtggggctg ttcctgcgtt gggcaggcgg
     4621 tctgcgcgtg gacagtgccg atgggctgca tgtggtgttg tatgactgct ctgtgggaca
     4681 tggggactgc agccgctgcc aaactgccat gcctcagtac gactgtgtgt ggtgtgaggg
     4741 ggagcgtccg cgttgtgtgg cccgggaagc ctgtaatgaa gccgagactg tggccactca
     4801 gtgccccgca cctctcattc actcggtgga tccactgact ggacctatag atggaggcac
     4861 ccgtgtcact atcaggggct ccaacctggg ccaacatgtg caggatgtcc tggacatggt
     4921 cagagtggcc ggagttccct gcgctgtgga tgctggggag tatgatgtct ctagtagtct
     4981 tgtgtgcatc actggagcca gcggggagga ggtgactggc actgtggcag tggaggtgcc
     5041 tggaagagga cacggtgtct cagagttcag ctttgcctat caggatccaa aagtacactc
     5101 catcttccca gcccgtggcc ctagagctgg aggtacccgc cttaccctgc atggttctaa
     5161 gctcctgact ggacggctag aggacatccg tgtggtggtt ggagaccagc cttgccacct
     5221 gctcctggag cagcagtctg agcagctaca ctgtgagacc ggcccatacc ctgtgcctgc
     5281 tgaacttcca gtgactgtct tgtttggggc cactgagcgg aggcttcagc acggccaatt
     5341 caagtataca tcagacccca atgtcacctc agtgggcccc tccaagagct tcttcagcgg
     5401 aggacgtgag atatgggtcc gcggccagga tcttgatgtg gtacagaggc caagaatccg
     5461 agtgaccgtg gtcccaagac agcatggcca ggggcttgca cagaagcaac acgtggtccc
     5521 tgagaaattt gaggagccgt gtctcgtgaa ctcctcccac ctcctcatgt gccgcactcc
     5581 cgctctccct ggcccaccct gggactctgg ggtccaggtg gagtttatcc tcgacaacat
     5641 ggtctttgac tttgctgcac tgagccccac acccttctcc tatgaggctg atcccaccct
     5701 gcgttccctg aaccccgagg atcccagcac gccgttccgg cacaagccag ggagtgtgtt
     5761 ctctgtggag ggggagaatc tggacctcgc catgtctaaa gaagaggtgg tggccatgat
     5821 aggggacggg ccctgcgtgg taaagacact gacccggaac cacctgtact gtgagccccc
     5881 tgtggagcag cccctgccac atccccatgc cctccgagag gctccagatg ctttgcctga
     5941 gttcacggta cagatgggca acctgcgctt ctccttgggt catgtgcagt acgatggcga
     6001 gagccccgtg gcttttcctg tggcaggcgc gcctgacaaa actcacacat gcccaccgtg
     6061 cccagcacct gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga
     6121 caccctcatg atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga
     6181 agaccctgag gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac
     6241 aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct
     6301 gcaccaggac tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc
     6361 agcccccatc gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta
     6421 caccctgccc ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt
     6481 caaaggcttc tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa
     6541 caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa
     6601 gctcaccgtg gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca
     6661 tgaggctctg cacaaccact acacacagaa gagcctctcc ctgtctccgg gtaaaggcgg
     6721 ccatcatcac catcaccatt aatctagagg atcttattaa agcagaactt gtttattgca
     6781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6841 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg
     6901 actctagcta gactcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7501 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7861 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7921 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7981 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8041 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8101 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8161 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8221 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8281 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8341 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8401 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8461 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8521 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8581 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8641 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8701 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8761 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8821 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8881 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     8941 aaacctctga cacatgcagc tcccggagga atgtgtgtca gttagggtgt ggaaagtccc
     9001 caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9121 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9241 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9301 aaaaagctcc cgggagcttg tatatccatt ttcgtcccgg agacggtcac agcttgtctg
     9361 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     9421 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     9481 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     9541 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     9601 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     9661 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     9721 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     9781 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     9841 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     9901 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     9961 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
    10141 att
//
