LOCUS       sequence-131363-        6617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Unc5A, isoform z protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Unc5a.z-Fc-His
FEATURES             Location/Qualifiers
     source          1..6617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3197..3214
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3240..3361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3277..3296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3331..3350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3399..3416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3570..4158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3650..3669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4329..5120,5121..5189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4952..4971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5190..5294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5362..5380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5418..5440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5442..5462)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5447..5777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5628..5763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5690..5709
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5914..5933
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6000..6455)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6137..6158)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6349..6368
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6581..6603
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6595..6612
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6596..6612
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggccgtccg
     1621 gcccggcctg tggccagcgc tcctgggcat agtcctcact gcctggcttc gtggttcggg
     1681 tgcccagcag agtgccacag tggccaaccc agtgcctggt gccaacccag acctgctgcc
     1741 ccacttcctg gtagagccgg aggacgtgta cattgtcaag aacaagcccg tgctgctggt
     1801 gtgcaaggct gtgcccgcca cccagatctt cttcaagtgc aacggggaat gggttcgcca
     1861 ggtcgatcac gtcatcgaac gcagcactga cggcagcagc ggattgccaa ccatggaggt
     1921 ccggatcaac gtatcaaggc agcaggtcga gaaagtgttt gggctggagg agtactggtg
     1981 ccagtgtgtg gcatggagct cctcaggaac caccaaaagc cagaaggcct acatccggat
     2041 tgcctatttg cgcaagaact ttgagcagga gccgctggcc aaggaagtgt cactggagca
     2101 aggcattgtg ctaccttgtc gccccccgga aggaatcccc ccagctgagg tggagtggct
     2161 ccgaaatgag gacctcgtgg acccctccct cgaccccaat gtgtacatca cacgggagca
     2221 cagcctagtc gtgcggcagg cccgcctggc cgacactgcc aactacacct gcgtggccaa
     2281 gaacatcgtg gcccgtcgcc gaagcgcctc tgcggccgtc attgtttatg tggatgggag
     2341 ctggagccca tggagtaagt ggtcagcctg cgggcttgac tgcacccact ggcggagccg
     2401 ggagtgctcc gacccagcgc cccgcaacgg aggtgaggag tgccggggtg ctgacctgga
     2461 cacccgcaac tgtaccagtg acctctgcct gcacacctct tccactagtg acaaaactca
     2521 cacatgccca ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
     2581 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt
     2641 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
     2701 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
     2761 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
     2821 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg
     2881 agaaccacag gtgtataccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
     2941 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa
     3001 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
     3061 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
     3121 atgctccgtg atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc
     3181 tccgggtaaa ggcggccatc atcaccatca ccattaatct agagatctta ttaaagcaga
     3241 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3361 atcatgtctg gtcgactcta gctagactct tccgcttcct cgctcactga ctcgctgcgc
     3421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     5341 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     5401 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aggaatgtgt gtcagttagg
     5461 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt
     5521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5701 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5761 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgtc ccggagacgg
     5821 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5881 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     5941 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     6001 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     6061 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     6121 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     6181 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     6241 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     6301 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     6361 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     6421 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     6481 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     6541 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     6601 acgacggcca gtgaatt
//
