LOCUS       sequence-13137-e        3636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1767-HQ858781
FEATURES             Location/Qualifiers
     source          1..3636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             181..201
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(1193..1292)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1309..1328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1310..1328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1333..1349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1333..1349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1451..1470)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1462..2271
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1529..1548)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2418..3006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2907..2926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3160..3177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3336..3363)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3455..3541)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3590..3612
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3604..3621
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3605..3621
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggtcgtc gtcgcgcccg ccgcctcctt ccaccacacg
      181 caccaccacc accaccacca cgaggccgcc gccgccgccg ccgcggctgc ggcggcctcc
      241 ggcgacccca tcttcccgct gctctccggc ggcccctgcg tgcttgatcc ggacaagtcg
      301 gcctccgcca gcgccatcca gttctggcag cagcagcctc cgccgccgtc gtcgccggct
      361 gctgcagcgg gcgctaaccc taaccctagc gccagcccct tcgcctacct caagaagccc
      421 ctcccgatgc tggacgccgg cgccggctcc tcaggctccg gcgccaccac gtgccaggac
      481 tgcggcaacc aagcgaagaa ggactgcggc cacaaccggt gccgcacctg ctgcaagagc
      541 cgcggcttcg actgcaacac gcacgtcaag agcacctggg tccccgccgc ccgccgccgc
      601 gagcgccagc agctcgccgc ctccggctcc gcctcgtcct cccccgccac cgcctccgcg
      661 gcggccgtcg ccgcgtccgc gtccgcgtcc gcgtccaaga agccccgcct cctctcgtcg
      721 cagaccacca cctcgcacac gtccacctcc aacgccacga cgccccgaag cttcgacacc
      781 acgtccagcc accaagacgc gtcgttcagg gacagcctcc cgaggcaggt gcgcgcgccg
      841 gcggtgttca agtgcgtgcg cgtgacgtcc atcgaggacg gcgaggacga gtacgcgtac
      901 caggcgacgg tcaccatcaa cggccacctc ttcaaggggt tcctgtacga ccagggcccc
      961 gacgacgggc gccacgcggc caccagcaac gacgactgca cggccggcgg ggtgcccaac
     1021 atctccgagc tccatctcgg agcggcgtct ggttccggcg ccggcaccgg cacctcgatg
     1081 tcgatggctc ctcccacggg gctgtacggt ggcggtggcg gaggacatca tccgcacatt
     1141 ctcggcgggt cgtcgagcta cggtaacacc atgaacaagg gtgggcgcgc cgacccagct
     1201 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1261 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1321 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1381 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1441 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1501 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1561 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1621 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1681 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1741 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1801 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1861 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1921 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1981 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2041 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2101 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2161 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2221 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2281 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2341 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2461 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2521 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2581 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2641 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2701 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2761 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     2821 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2881 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2941 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3001 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3061 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3181 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3241 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3301 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3361 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3421 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3481 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3541 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3601 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
