LOCUS       sequence-131377-        8028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express full-length Flrt1 with a C-terminal myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: pTT3 Flrt1-myc
FEATURES             Location/Qualifiers
     source          1..8028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2043..2072
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2123..2178
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2177..2196)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4414..4432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4500..4604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4605..4673,4674..5465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4823..4842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5636..6224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6125..6144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6378..6395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6512..6533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6548..6553,6554..6571,6572..6578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6586..6602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6591..6613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6610..6626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6610..6626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6897..7276
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7277..7480
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7431..7451
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7477..7501
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7982..8001
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcggccgcgc caccatggtg gtggcacact ctgctgccac cgctaccacc acacctgctg
       61 ccacggtcac agccactgtc gtgatgacca cagccaccat ggacctgcgg gactggctgt
      121 ttctctgtta tgggctcatt gccttcctca cggaggtcat cgatagcacc acctgcccat
      181 cagtgtgccg ctgtgacaat ggcttcatct actgcaatga ccggggactc acatccatcc
      241 cctcagacat ccccgacgat gccaccaccc tctacctaca gaacaaccag atcaacaatg
      301 cgggcatccc tcaggacctc aagaccaagg tcaaggtgca ggtcatctac ctgtatgaga
      361 atgacctgga cgagttccct atcaatctcc cccgctccct gagagagctg catctgcagg
      421 acaacaatgt gcgcaccatc gccagggact ctttggctcg catcccgctg ctggagaagc
      481 tgcacctgga cgacaactcc gtttccaccg tgagcatcga ggaggatgct tttgctgaca
      541 gcaagcagct caagctgctt ttcctgagcc ggaaccacct gagcagcatc ccgtcagggc
      601 tgccccacac gctggaggaa ctgaggctag atgacaaccg catctctacc atccccctgc
      661 acgcattcaa gggtctcaac agcctgcggc gcctggttct ggatggcaac ctgctggcca
      721 accagcgcat tgccgacgac accttcagcc ggctgcagaa cctcacggag ctgtcgctag
      781 tacggaactc gctggctgcc ccgcccctta atctgcccag cgcccacctg cagaagctct
      841 acctgcagga caatgccatc agtcacattc cctacaacac cctggccaag atgcgggagc
      901 tggagaggtt ggacctgtcc aacaacaacc tcaccacgct gcctcggggc ctgtttgatg
      961 acctggggaa cctggcacag ctgcttctca ggaacaaccc ctggttctgt ggctgtaacc
     1021 tcatgtggct gagggactgg gtgagggcac gggctgcagt ggtcaatgtg cggggcctca
     1081 tgtgccaggg ccctgagaag gtccggggca tggccatcaa agacatcacc agcgagatgg
     1141 atgagtgctt tgaggcgggg tcacagggcg gtgctgctaa tgcagccgcc aagaccacag
     1201 tcagcaacca tgcctctgcc accacacccc agggctctct gtttaccctc aaggccaaga
     1261 ggccaggact gcgcctccca gactccaaca ttgactaccc catggccact ggcgatggcg
     1321 ccaagacatt ggtcatccag gtgaagccac tgacggcaga ctctatccga atcacatgga
     1381 aggccatgct acccgcctct tctttccggc tcagttggct acgtctgggc catagcccgg
     1441 ctgtgggctc tataacagag accctggtgc agggggacaa gacagagtac ttgctgacag
     1501 cactggagcc caagtccacc tacatcatct gtatggtcac catggagact ggcaacacct
     1561 acgtggccga tgagacacct gtgtgtgcca aggcagagac agcagatagc tatggcccta
     1621 ccaccacgct caaccaggaa cagaatgctg gccctatggc ggggctgccc ctggctggga
     1681 ttattggtgg tgccgtggct cttgtatttc tcttcctggt cctgggagcc atttgctggt
     1741 acgtgcaccg ggctggcgag ctgctgaccc gagagagggt ctacaacagg ggcagcagga
     1801 ggaaggacga ctacatggag tcagggacca agaaggataa ctccattcta gaaatccgtg
     1861 gcccaggact gcagatgcta cccatcaacc cgtaccgctc caaagaggag tacgtggtgc
     1921 acaccatatt tccctccaat ggcagcagcc tctgcaaggg tgcccacact attggctatg
     1981 gcaccacacg aggctaccgg gaagcgggca tccctgatgt ggactactcc tacacaggcg
     2041 gcgagcagaa gctgatcagc gaggaggatc tgtaaactag acacgtgatt aattaaggat
     2101 cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     2161 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt ggtcgagatc
     2221 cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
     2281 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     2341 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     2401 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     2461 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     2521 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     2581 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     2641 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     2701 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     2761 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     2821 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     2881 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     2941 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     3001 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     3061 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     3121 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     3181 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     3241 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     3301 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     3361 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     3421 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     3481 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     3541 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     3601 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     3661 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     3721 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     3781 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     3841 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     3901 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     3961 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     4021 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     4081 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     4141 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     4201 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     4261 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4321 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgataagct
     4381 gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     4441 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     4861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     5161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6601 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacc
     6661 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
     6721 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
     6781 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     6841 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
     6901 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     6961 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     7021 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     7081 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     7141 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     7201 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
     7261 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
     7321 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     7381 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
     7441 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     7501 cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc ggttgaggac
     7561 aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct ccgaacggta
     7621 ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac ctctcgagaa
     7681 aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggt
     7741 ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag taggcggtct
     7801 tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg ttggggtgag
     7861 tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc caaaaacgag
     7921 gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa tgacatccac
     7981 tttgcctttc tctccacagg tgtccactcc caggtccaag tttaaact
//
