LOCUS       sequence-131378-        7986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express full-length Flrt2with a C-terminal myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: pTT3 Flrt2-myc
FEATURES             Location/Qualifiers
     source          1..7986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2001..2030
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2081..2136
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2135..2154)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4372..4390)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4458..4562
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4563..4631,4632..5423)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4781..4800)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5594..6182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6083..6102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6336..6353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6470..6491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6506..6511,6512..6529,6530..6536)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6544..6560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6549..6571
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6568..6584
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6568..6584
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6855..7234
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7235..7438
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7389..7409
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7435..7459
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7940..7959
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcggccgcgc caccatgggc ctacagacta caaagtggcc cggccgtggg gctttcatcc
       61 tcaaattttg gctcatcatt tccctgggac tctacttaca agtgtcaaaa ctcctggcct
      121 gccctagtgt atgccgctgt gacaggaact ttgtctactg taacgagaga agcttgacct
      181 cagtgcctct tgggatcccg gagggcgtaa ccgtactcta cctccacaac aaccaaatta
      241 ataatgctgg ctttcctgca gagctgcaca atgtccagtc agtgcacact gtctaccttt
      301 atggcaacca gttggatgag ttccccatga accttcccaa gaatgtcaga gttctccatc
      361 tgcaggaaaa caacattcag accatctcgc gggctgctct cgctcagctc ctcaagctgg
      421 aagaactcca cctggacgac aactccatat ctacagtggg agtagaagac ggagcgttcc
      481 gggaggcgat tagcctcaaa ctgttgtttt tatcgaagaa tcacctgagt agcgtgcctg
      541 ttgggcttcc tgtagacttg caagagctga gagtggatga aaaccgaatt gccgtcatat
      601 cagacatggc ctttcagaac ctcacaagct tggagcgcct gatcgtggat gggaatcttc
      661 tgaccaacaa gggcattgct gagggtacct tcagtcatct caccaagctc aaggaatttt
      721 ctatagtccg gaactcgctc tcccacccac ctcctgatct cccaggtacg catctgatca
      781 ggctctactt gcaggataac cagataaacc atatcccatt gacagccttc gcaaacctcc
      841 gtaagctgga aaggctagat atatccaaca accagctacg aatgttaact caaggagtct
      901 tcgatcatct ctccaacctg aaacaactca ctgcgcggaa taacccttgg ttttgtgact
      961 gcagcattaa atgggtcaca gaatggctca agtatatccc ttcttctctc aacgtgcgtg
     1021 gtttcatgtg ccaaggtcct gagcaagtcc gcgggatggc tgtcagggag ttgaatatga
     1081 atcttttgtc ttgtcccacc acgactcctg gcctacctgt ctttacccca gctccaagta
     1141 ccgtttctcc aacaactcag tctcctactc tctctgttcc aagccccagc agaggctctg
     1201 tgcctccagc tcctacccca tcgaaacttc ccaccatccc tgattgggat ggcagagaaa
     1261 gagtgacccc acctatttct gaaaggatcc aactttccat ccactttgtt aatgatactt
     1321 cgattcaagt cagctggctg tctctcttta ctgtgatggc ctacaaactg acatgggtga
     1381 aaatgggcca cagtctcgta gggggcatcg ttcaggaacg aattgtcagt ggtgagaagc
     1441 aacacctgag cttggttaat ttagagccca gatccacgta taggatttgt ttagtgccgc
     1501 tggatgcgtt caactaccgc actgtggaag ataccatctg ttcggaggct accacccatg
     1561 cctcttattt gaacaacggc agcaacactg cttctagcca tgagcagacg acttcccaca
     1621 gtatgggctc cccttttctg ctcgcaggct tgattggggg cgcagtgatt tttgtgctcg
     1681 ttgtcttgct cagcgtcttt tgctggcaca tgcacaaaaa gggacgctac acctcccaga
     1741 agtggaaata caaccggggc cgacggaaag acgactattg tgaagcgggt accaaaaaag
     1801 acaactccat cttggagatg acagaaacaa gttttcagat tgtctcctta aataacgatc
     1861 agctccttaa aggagatttc agactgcagc ccatttatac cccaaatggg ggcattaatt
     1921 acacagactg ccacatcccc aacaacatga gatactgcaa cagcagtgtg ccagatctag
     1981 agcattgcca tacgggcggc gagcagaagc tgatcagcga ggaggatctg taaactagac
     2041 acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa atttattttc
     2101 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2161 aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac
     2221 gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct
     2281 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     2341 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     2401 ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac
     2461 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     2521 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     2581 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     2641 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     2701 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     2761 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     2821 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     2881 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     2941 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     3001 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     3061 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     3121 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     3181 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     3241 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     3301 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     3361 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     3421 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     3481 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     3541 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     3601 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     3661 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     3721 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     3781 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3841 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     3901 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     3961 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
     4021 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
     4081 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
     4141 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4201 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4261 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     4321 ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga
     4381 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4441 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4501 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4561 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4621 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4681 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4741 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4801 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4861 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4921 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4981 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5041 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5101 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5161 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5221 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5281 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5341 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5401 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5461 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5521 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5701 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6301 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6361 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6421 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6481 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6541 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6601 tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     6661 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     6721 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     6781 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     6841 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     6901 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     6961 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     7021 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     7081 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     7141 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     7201 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     7261 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     7321 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     7381 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     7441 tttagtgaac cgtcagatcc tcactctctt ccgcatcgct gtctgcgagg gccagctgtt
     7501 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     7561 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     7621 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     7681 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     7741 aattaaagta ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga
     7801 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     7861 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     7921 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt
     7981 taaact
//
