LOCUS       sequence-13140-e        5163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1770-HQ858784
FEATURES             Location/Qualifiers
     source          1..5163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2720..2819)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2836..2855)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2837..2855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2860..2876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2860..2876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2978..2997)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2989..3798
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3056..3075)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3945..4533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4434..4453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4687..4704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4863..4890)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4982..5068)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5117..5139
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5131..5148
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5132..5148
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcggcg gtggcgagca gggagaggct ctccccgggc
      181 gccgcgccgc aggtggacgc gggcaagtac gtgcggtaca cgccggagca ggtggaggca
      241 ctggagcgcg tctacagcga gtgccccaag cccagctcgc tgcgccggca gcacctcatc
      301 agggactgcc ccatcctccg caacatcgag cccaagcaga tcaaggtctg gttccagaac
      361 cgcagatgcc gggagaagca gcgcaaggag tcctctcgtc tacaaaccgt gaaccggaag
      421 ctgagtgcga tgaacaagct gttgatggag gagaatgacc ggctgcagaa gcaggtttcc
      481 cgtctcgtct ttgacaacgg gtatatgaag aatcgactcc acagtccttc tgtagccacc
      541 accgacacaa gctgtgagtc tgtggtgaca agtggtcagc acaaccaaca gcaaaaccca
      601 gcagttctgc atcctccaca aagggatgcg aacaacccag caggtctact cgctattgct
      661 gaggagacat tggcagagtt catgtccaag gcgactggaa ctgctgtcaa ctgggtgcaa
      721 atggttggga tgaagcctgg tccggattcc gttggaatca tcgctgtttc gcacaactgc
      781 agcggcgtag cagcacgagc ttgcggcctt gtgagccttg agcccacaaa ggttgctgag
      841 atccttaagg atcgtgcatc gtggtatcgc gattgtcggc atgttgatgt cctccatgtt
      901 atccctacgg gtaacggtgg aacgattgag ctgatctata tgcagactta tgcactgaca
      961 actctcgcgg aaccacgaga cttttggacg ctccgataca ctagtggtct tgacgatggc
     1021 agtcttgtga tctgcgaaag gtcattgact cagtccactg gaggtccttc tggacctaac
     1081 actccaaatt ttatcagagc tgaggtgctt cctagtggtt atctgattcg accctgtgat
     1141 gggggaggtt ccatgattta cattgtggat catgttaatc tgaatgcttg tagtgtgcct
     1201 gaggttcttc ggccactcta tgaatcccct aagatactgg cacagaagat gactgcggcg
     1261 gcgttgcgac acattaggca aattgcacat gagtcaagtg gtgaaatgcc ctatggtgct
     1321 gggcgacagc cggctgttct cagaactttc agtcaaaggc tcagcagagg cttcaatgat
     1381 gctgtgagtg gctttccaga tgatggctgg tcttgtttgt tgaacaccga tggtgctgag
     1441 gatattacag ttacaatcaa ctcatctcca aataaacttg ttgggtctca tgtcagcgct
     1501 tccccattat tttctgctat ggggggtgga atcatgtgcg caaaagcatc gatgctgctg
     1561 cagaatgtgc cacctgctct acttgtgcga tttttgaggg agcatcggtc tgaatgggct
     1621 gatcctggca tcgatgctta ttctgtcgcc tctttgaggg ccaacccata cactgttcca
     1681 ggtttaaggg ctggtgggtt tatgggcaac caggttatac tgccccttgc acgcaccttg
     1741 gagcatgaag agtccttgga ggttattagg cttgaaggac atggctttag ccatgaggag
     1801 gtgcttatgt cccgggatat gtttcttcta cagctgtgca gtggcgtcga cgaggatgca
     1861 cctggtgcgt gtgcacagct tgtctttgct cctattgatg aatcttttgc cgacgatgca
     1921 ccgttgctgc cctcaggctt ccgtgtgata ccgcttgatg ctaagacgga tgtgccaacg
     1981 gccaccacac gcacgcttga ccttgcctct gcgctcgagg ttggatcagg tggaagcggc
     2041 atgcgcgctt catgcgatgg ttccggcacg tgcgccacga gatcggtcct gaccatcgcc
     2101 ttccagttct cgttcgagaa ccaccttcgc gagagcgtgg cggcaatggc caagcagtac
     2161 gtcaggggcg tgatggcctc cgtgcagagg gtggccatgg cgatcgctcc ttctcgcctc
     2221 ggttcgcgta tcgaactgaa gcatccgccg ggctctcccg aggcacttgc actagctacg
     2281 tggattggca ggagctacag ggcgcacact ggaacggaga tccgctggtc ggacaccgaa
     2341 ggcgcggact ctccactgat gctgttctgg aagcacagcg acgcgatact ctgctgctct
     2401 ctgaagccgc cggctttcac gctcaagttc gccaacagcg ccggcttcga catcctggag
     2461 acgacgatgg tgaacatcca ggacctgccg ctggaggcgg tcctcgacga ggagggccag
     2521 aaggccctgt ccgcgcagct ccccgcgatc atgcagcagg gcctggcgta cctccccggt
     2581 ggcgtgtgca ggtcgagcat ggggcggcag gcgtcgtacg agcaggcagt ggcgtggaag
     2641 gtggtgggtg acgacggcgc cccgcagtgc ctggcgctca tgctcgccaa ctggaccttc
     2701 atcaagggtg ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     2761 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     2821 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     2881 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2941 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     3001 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     3061 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     3121 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     3181 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     3241 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     3301 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     3361 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     3421 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     3481 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3541 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     3601 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3661 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3721 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3781 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3841 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4081 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4321 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4621 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4801 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4861 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4921 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4981 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     5041 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     5101 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     5161 ccc
//
