LOCUS       sequence-131404-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of TRP120 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Luo T, Zhang X, McBride JW
  TITLE     Major species-specific antibody epitopes of the Ehrlichia 
            chaffeensis p120 and E. canis p140 orthologs in surface-exposed 
            tandem repeat regions.
  JOURNAL   Clin Vaccine Immunol. 2009 Jul;16(7):982-90. doi: 
            10.1128/CVI.00048-09. Epub 2009 May 6.
  PUBMED    19420187
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/CVI.00048-09"; journalName: "Clin Vaccine Immunol"; date: 
            "2009-07"; volume: "16"; issue: "7"; pages: "982-90"
COMMENT     Sequence Label: pBAD-TRP120
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             85..414
                     /codon_start=1
                     /product="His-Patch thioredoxin"
                     /label=HP-thioredoxin
                     /note="The histidines at positions 8, 32, and 64 coordinate
                     a divalent cation, allowing HP-thioredoxin to be purified 
                     on metal-chelating resins."
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEF
                     LDANLA"
     CDS             430..444
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat acccatggga tctgataaaa ttattcatct gactgatgat
      121 tcttttgata ctgatgtact taaggcagat ggtgcaatcc tggttgattt ctgggcacac
      181 tggtgcggtc cgtgcaaaat gatcgctccg attctggatg aaatcgctga cgaatatcag
      241 ggcaaactga ccgttgcaaa actgaacatc gatcacaacc cgggcactgc gccgaaatat
      301 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
      361 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccggctct
      421 ggatccggtg atgacgatga caagctcgcc cttatggata ttgataatag taacataagt
      481 acagccgata tacggagtaa tactgatggc ttgatagaca taattatgcg tatattaggt
      541 tttggtaata agaatattgt gcaaccacag gatctgggtt ctgaaattta tcagcaagag
      601 caagaagatg acacagtctc tcaaccttca ttagagccat ttgttgcaga aagtgaagtt
      661 tctaaagttg aacaagaaa
//
