LOCUS       sequence-131405-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV113.loxP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..305
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 agagcttggt gagcgctagg agtcactgcc aggtatcgtt tgaacacggc attagtcagg
       61 gaagtcataa cacagtcctt tcccgcaatt ttctttttct attactcttg gcctcctcta
      121 gtacactcta tattttttta tgcctcggta atgattttca tttttttttt tccacctagc
      181 ggatgactct ttttttttct tagcgattgg cattatcaca taatgaatta tacattatat
      241 aaagtaatgt gatttcttcg aagaatatac taaaaaatga gcaggcaaga taaacgaagg
      301 caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc aagattcaga
      361 ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc ccagaaaaag
      421 aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc cacacaggta
      481 tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg tcgctaatcg
      541 ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac tgcgggattg
      601 ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa aggtttggat
      661 caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt tcgaacaggc
      721 cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga
      781 tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc cacgttgatt
      841 gtctgcgagg caagaatgat catcacc
//
