LOCUS       sequence-131407-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C 
            Virus (HCV) infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Ren Q, Li C, Yuan P, Cai C, Zhang L, Luo GG, Wei W
  TITLE     A Dual-Reporter System for Real-Time Monitoring and High-throughput 
            CRISPR/Cas9 Library Screening of the Hepatitis C Virus.
  JOURNAL   Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038/srep08865.
  PUBMED    25746010
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-03-9"; volume: "5:8865. doi"; pages: " 
            10.1038/srep08865"
COMMENT     Sequence Label: pLenti-NIrD
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(124..177)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccctt
       61 taagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccattct
      121 agaggggccg gggttctcct ccacgtcgcc gcaggtcagc aggctgcctc tgccctcgcc
      181 gctgccgaat tcgttagcct cccccatctc ccgggcaaac gtgcgcgcca ggtcgcagat
      241 cgtcggtatg gagccggggg tggtgacgtg ggtctggacc atcccggagg taagttgcag
      301 cagggcgtcc cggcagccgg cgggcgattg gtcgtaatcc aggataaaga cgtgcatggg
      361 acggaggcgt ttggccaaga cgtccaaggc ccaggcaaac acgttgtaca ggtcgccgtt
      421 gggggccagc aactcggggg cccgaaacag ggtaaataac gtgtccccga tatggggtcg
      481 tgggcccgcg ttgctctggg gctcggcacc ctggggcggc acggccgtcc ccgaaagctg
      541 tccccaatcc tcccgccacg acccgccgcc ctgcagatac cgcaccgtat tggcaagcag
      601 cccgtaaacg cggcgaatcg cggccagcat agccaggtca agccgctcgc cggggcgctg
      661 gcgtttggcc aggcggtcga tgtgtctgtc ctccggaagg gcccccaaca cgatgtttgt
      721 gccgggcaag gtcggcggga tgagggccac gaacgccagc acggcctggg gggtcatgct
      781 gcccataagg tatcgcgcgg ccgggtagca acaggaaggg cggcgatggg atggcggtcg
      841 aaaatgaagg tgagggccgg gggccggggc atgtgagctc ccagccttcc cccccgatat
      901 gaggaagcca gaacggcgtc gggtcacggc ataaggcatg cccatttgtt atctgggcgc
      961 ttgt
//
