LOCUS       sequence-131410-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of TRP120 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Luo T, Zhang X, McBride JW
  TITLE     Major species-specific antibody epitopes of the Ehrlichia 
            chaffeensis p120 and E. canis p140 orthologs in surface-exposed 
            tandem repeat regions.
  JOURNAL   Clin Vaccine Immunol. 2009 Jul;16(7):982-90. doi: 
            10.1128/CVI.00048-09. Epub 2009 May 6.
  PUBMED    19420187
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/CVI.00048-09"; journalName: "Clin Vaccine Immunol"; date: 
            "2009-07"; volume: "16"; issue: "7"; pages: "982-90"
COMMENT     Sequence Label: pBAD-TRP120
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..89)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tggagaccgt ttaaactcaa tggtgatggt gatgatgacc ggtacgcggt agaatcgaga
       61 ccgaggagag ggttagggat aggcttacct tcaagctcgc cctttacaat atcatttact
      121 acattgtgat taatattctc atggttagta aagtaattat gttccataaa ataaaggcta
      181 acattacaac acacaggaac attacttaat aaaccaccta ataagtcttt cacatcttga
      241 aatccataca tatacatttc atacccacaa acatactgtc caggttttga cataggacat
      301 aacattccac tttcaaatat tgttgaaact atagaatcca tactttctaa gcattcgata
      361 ggtgatatta tatcttcact accttttaca gtctgtgaat cctttacaat ctcaaattct
      421 tgttcgcaag tttcacaaac tttatcttcc tcctttataa cgattggatt aaatgaaggt
      481 gcaaacattt catctttctc tacaacaacc ggaatcactt gacctaatgg caaatcttct
      541 actagaattt cagggttagt tttttcttgt tcaactttag aaacttcact ttctgcaaca
      601 aatggctctg atgaaggttg agatactatt tcatcttctt tctgatgaga ttcagttatt
      661 ccactttctt tttctgtttc tccttgatgg gattcaattt cactttctct ctctgtaaca
      721 acttgagctg gctgatctga tacaccagat tcatgactcg caacatcttg caaatctttt
      781 attaa
//
