LOCUS       sequence-131413-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For making transgenic fish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Bertrand JY, Chi NC, Santoso B, Teng S, Stainier DY, Traver D
  TITLE     Haematopoietic stem cells derive directly from aortic endothelium 
            during development.
  JOURNAL   Nature. 2010 Mar 4;464(7285):108-11. doi: 10.1038/nature08738. Epub 
            2010 Feb 14.
  PUBMED    20154733
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08738"; journalName: "Nature"; date: "2010-03-4- 4"; 
            volume: "464"; issue: "7285"; pages: "108-11"
COMMENT     Sequence Label: bactin2:loxP-STOP-loxP-DsRed-express
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          76..142
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             272..292
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    717..798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(753..772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggatctttgt gaggacctta cttctgtggt gtgacataat tggacaaact acctacagag
       61 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aactgtgtta aactactgat
      121 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
      181 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
      241 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
      301 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
      361 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
      421 tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc ataacatact
      481 gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt
      541 gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc
      601 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      661 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      721 tgtttattgc agcttataat ggttacaata aagcaatagc atcacaaatt tcacaaataa
      781 agcatttttt ttcactgcat tctagttgtg g
//
