LOCUS       sequence-13142-e        3597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1800-HQ858786
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1154..1253)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1270..1289)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1271..1289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1294..1310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1294..1310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1412..1431)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1423..2232
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1490..1509)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2379..2967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2868..2887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3121..3138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3297..3324)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3416..3502)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3551..3573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3565..3582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3566..3582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggagtgg acggccccga agcccgcctc atcgcctccc
      181 ctcctctggg actggggaga ccacgccgcc acgggctcgg gctcctccag cgacgccccg
      241 gcgcggcgcg gcgggaagga gcgggaggcg aagcgtgcca gggccggcga ggaccgcggg
      301 ggaggtgagc tgaggtgcca ggtcgagggg tgcggattgg acctcagcag ggtcaaggac
      361 taccaccgta agcaccgcgt ctgcgaggcc cacaccaagt ccccccgcgt catcgtcgcc
      421 ggccaggagc gccggttctg ccagcagtgc agccgattcc atgcgctgtc agagtttgat
      481 cagaagaaga ggagctgcag gaggcgtctg tctgatcaca atgcccgccg ccggaagcct
      541 cagccagatg cattcacctt cgcctctgca aagctgcctt cgacattgtt tgatgatagg
      601 cggcaaataa gttttgtctg gaataaagct cctgttagcc atgtaagacc cttcacttct
      661 ccatgggaca gctcatctga cttaaagccc ccttatgcaa aggaaataag tgatgtatca
      721 acaaaagttg ggacaattac tggacaagtt catttggata aatctcacat gttcaatgct
      781 attccaacac ttagccatgg caaagatgag ccttggccaa tgaaaggtct ggacatgtct
      841 atatctgctt caaaattcga tggagcagcg gatcttcagc atgctctctc tcttctgtca
      901 gccggcgctt gtggactgcc tgattctgta catcaaacat cttgcattat ccaattcaat
      961 ggtgccagcg agaacagcag tgaccttcat gtaacgcatg gcaggaactc tggtccagca
     1021 tcatgcgccg atgcgcagca tatagctgcc cagcctcagt ctcagctgtt tcattttacc
     1081 acagataccg gcaatactgt ttatgagccc agtttctttg gtgtaaacca gataaataag
     1141 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1201 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1261 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1321 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1381 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1441 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1501 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1561 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1621 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1681 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1741 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1801 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1861 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1921 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     1981 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2041 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2101 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2161 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2221 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2281 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2521 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2761 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3181 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3241 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3301 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3361 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3421 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3481 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3541 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
