LOCUS       sequence-13144-e        4428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1802-HQ858788
FEATURES             Location/Qualifiers
     source          1..4428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1985..2084)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2101..2120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2102..2120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2125..2141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2125..2141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2243..2262)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2254..3063
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2321..2340)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3210..3798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3699..3718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3952..3969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4128..4155)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4247..4333)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4382..4404
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4396..4413
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4397..4413
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggatcaa cttgccctgc ttgcgacgga gctgggtgac
      181 tcgtcagact ttgaggtgga gggcatccag aacctcactg agaacgatgt cagtgacgag
      241 gagattgagc ccgaggacct ggcgcggcga atgtggaagg acagagtcag gctgaggagg
      301 atcaaggagc ggcagcacaa gcttgccctg cagcaggcag agctgcagaa gttgaggccc
      361 aagcccatat ccgaccaggc catgcgcaag aagatgtcaa gggcacacga cgggatcctc
      421 aagtatatgc tcaagctcat gcaggtgtgc aatgcacgcg ggttcgttta tgggatcatc
      481 ccggacaagg ggaagcctgt cagtggcgct tccgataata tcagagcttg gtggaaggag
      541 aaggtgaagt ttgataagaa cgggcctgcg gcgattgaga aatacgagtc tgacaactta
      601 gtatccgcta ctgcccagag tggtggaact aagagccagc acagcttgat ggatctccaa
      661 gatgccactc ttggttcact gctttcctca ttgatgcagc attgtgatcc accgcagcgc
      721 aagtacccat tggagaaggg tgcttcaccc ccgtggtggc cttcagggaa ggaggaatgg
      781 tggaccgccc tgggtcttcc aagcggccaa gttcctcctt acaagaaacc acatgatctt
      841 aagaaggtct ggaaggctgg tgtgcttaca ggtgtaatca agcacatgtc tcccaatttt
      901 gataagataa ggaatcatgt acggaaatca aagtgtttgc aggataaaat gactgcaaaa
      961 gaaagcctga tttggctggg agttctacag agagaagaaa gccttgttca cagaatcgac
     1021 aatggcgtat cggagattac tcagcgcagt atgccagagg agaggattgc agacacaaat
     1081 agcagcagta atgagtatga tgtgtatggt tttgaggatg ctcctgtttc tacatcatct
     1141 aaagatgatg aacaagatct gtctccagtt gcacaatctg ctgtggagca tgttccgaaa
     1201 agaggaaggg aaagagctta caataaacgc cctaatcaaa tcgttcctgg taaggcaaaa
     1261 gaaccaccaa agagaaaaag aacacgccac agctccactg ttaccgagcc agatgcacac
     1321 agagttgttg atgcaccaga aaactcaaga aatttgatcc ctgatatgaa tagattggat
     1381 caagtagaaa tcccaggcac gtcaaaccag attgtcacct ttgaccatgg gggcaccaca
     1441 actgaagctt tacaacacag aggagatgct caagtacagg tccatcctcc tggtgctgag
     1501 gttaatagct tctatagtgc tccaactgca aaccctactc ctataagcat atatatgggt
     1561 gaccagccta taccttatca aaataatgac agcacaaggc cgagatctga aaatagtttt
     1621 cctgtagatg ctcatactgg tttaaataat cttcccagca gttatcagaa cttacctccg
     1681 aaacaatcac taccacttcc catgatggat catcatgtgg ttcccatggg tatcagggca
     1741 cctactgata gcattcctta cggtgatcat atactaggtg gtggaaattc aacttctgtt
     1801 cctggagata tgcagcaact catagatttt cctttctatg ctgaacaaga taagtttgtt
     1861 ggcagttcct ttgaggggtt gcctttagac tatatcagta tcagtagtcc aatcccagat
     1921 attgatgact tgctgcatga tgatgattta atggaatact tgggaacaaa gggtgggcgc
     1981 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2041 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     2101 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     2161 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     2221 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     2281 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2341 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2401 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2461 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2521 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2581 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2641 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2701 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2761 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2821 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2881 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2941 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3001 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3061 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3121 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3601 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4021 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4081 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     4141 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     4201 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     4261 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     4321 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     4381 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
