LOCUS       sequence-131445-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Biomedical Research.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Dasgupta S, Hoque MO, Upadhyay S, Sidransky D
  TITLE     Mitochondrial cytochrome B gene mutation promotes tumor growth in 
            bladder cancer.
  JOURNAL   Cancer Res. 2008 Feb 1;68(3):700-6. doi: 
            10.1158/0008-5472.CAN-07-5532.
  PUBMED    18245469
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/0008-5472";
            journalName: "Cancer Res"; date: "2008-02-1- 1"; volume: "68"; 
            issue: "3"; pages: "700-6"
COMMENT     Sequence Label: pCMV-myc-mito
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..125
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             174..203
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(239..256)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    245..469
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      515..943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(602..621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     812..833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(952..972)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcagagcttt ctggctaact agagaaccca ctgcttactg gcacgtgacc atgtccgtcc
       61 tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca gtgccgcgcg
      121 ccaagatcca ttcgttgctg caggtcgacc tcgagatcaa acgggcggcc gcagaacaaa
      181 aactcatctc agaagaggat ctgaatgggg ccgcatagtc tagaagctcg ctgatcagcc
      241 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      301 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
      361 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
      421 gattgggaag acaatagcag gcatgctggg gatggcccgg gctctatggc ttctgaggcg
      481 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
      541 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      601 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      661 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct ctcccccaaa
      721 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      781 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
      841 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
      901 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
      961 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1021 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagc
//
