LOCUS       sequence-131458-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T1ER is a calcium sensor targeted to ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Bandara S, Malmersjo S, Meyer T
  TITLE     Regulators of Calcium Homeostasis Identified by Inference of Kinetic
            Model Parameters from Live Single Cells Perturbed by siRNA.
  JOURNAL   Sci Signal. 2013 Jul 9;6(283):ra56. doi: 10.1126/scisignal.2003649.
  PUBMED    23838183
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-9"; volume: "6(283):ra56. doi"; pages: " 
            10.1126/scisignal.2003649"
COMMENT     Sequence Label: pcDNA-T1ER
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(119..121,122..634)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(161..182)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(422..441)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     769..790
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttatg ctgctgcccg tccccctgct gctgggcctg ctgggcgccg ccgccgacgt
      121 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      181 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      241 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      301 gaccaccctg tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
      361 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      421 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      481 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      541 ggagtacaac tacatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
      601 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
      661 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      721 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      781 ggagttcgtg accgccgccc gcatgcatga ccaactgaca gaagagcaga ttgcaaaatt
      841 caaagaagcc ttctcattat tcgacaagga tggggacggc accatcacca caaaggaact
      901 tggcaccgtt atgaggtcgc tt
//
