LOCUS       sequence-13146-e        3792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1804-HQ858790
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             1082..1090
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(1349..1448)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1465..1484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1466..1484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1489..1505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1489..1505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1607..1626)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1618..2427
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1685..1704)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3492..3519)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3611..3697)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3746..3768
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3760..3777
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3761..3777
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggagggg atggaggagg ccaacaggac ggcagtggag
      181 agctgccacc gggtgctggc gctcctctcc aacccgcacg gccagctcgt ccccagcaag
      241 gagctcgtgg ccgccaccgg ggaggccgtc gccaagttcg gctcactgac ggccaagctc
      301 tccaactcca acggcgatgg cctactgcag ggccacgcta gggtcaggaa ggtcaagaag
      361 cccctgcaca tcttcgacag caacctcttc ctcgagagct ctgcggtggc cgccgccgct
      421 gctccggcca agacgcccag cccgagcccg atccttggcc tccagctgtt cccgaggtac
      481 caccagtttg agggctcgtc gtctaaggat cctgtcagga tccctaccca gttccccaag
      541 aggttgctgc tagagaagcc gactgccggt atggaggggt cgacgtcgca gtcccctccg
      601 atcgtccaga tggtccagcc ggtgtccgtt gcgccccccg cagggacgcc caccccggca
      661 ttgccccccg ctcaccttca tttcatccag cagcagcaaa gctaccagag gtttcagctc
      721 atgcagcaga tgaagatcca gagcgagatg atgaagagga gcaatcttgg tgatcagggt
      781 ggtagcttaa gtggcggcgg cggcggcggt cgtaagggtg tgaatctcaa gtttgacagc
      841 tctaattgca cggcgtcatc gtctcgctcg ttcctttcgt ctctgagcat ggaagggagt
      901 cttgcgagtt tggatggaag ccggaccagc aggccgttcc agctacttag tggctctcag
      961 acagctagca cgccggagct gggcctggtg caaaggagaa ggtgcgctgg cagggaggat
     1021 gggactggtc ggtgtgcaac cgggagccgg tgccattgtt caaagaaaag gaagctaagg
     1081 ataaggaggt ccatcaaagt ccctgcaata agcaacaagg ttgcagacat cccagctgat
     1141 gaattctcat ggaggaagta tgggcagaag ccaattaagg gatccccaca tcctaggggt
     1201 tattacaagt gtagcagtgt gagaggctgc cccgcgagga agcatgtcga gaggtgcgtg
     1261 gacgacccct cgatgctgat tgttacctat gaaggtgacc acaaccacaa ccgagttcta
     1321 gcccagccag ccaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata
     1381 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1441 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1501 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1561 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1621 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1681 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1741 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1801 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1861 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1921 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1981 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     2041 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     2101 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     2161 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2221 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2281 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2341 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2401 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2461 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2521 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3481 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3541 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3601 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3661 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3721 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3781 aagctcgggc cc
//
