LOCUS       sequence-131460-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV113.loxP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..50
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     CDS             complement(130..807)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             814..825
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gtcgacggca gctgggataa cttcgtataa tgtacattat acgaagttat gggacgtggc
       61 gaattcctgc agcccccagt agagaccgcc tggctctagt agcgatctac actagcacta
      121 tcagcgttat taagcaccgg tggagtgacg accttcagca cgttcgtact gttcaacgat
      181 ggtgtagtct tcgttgtggg aggtgatgtc cagtttgatg tcggttttgt aagcacccgg
      241 cagctgaacc ggttttttag ccatgtaggt ggttttaact tcagcgtcgt agtgaccacc
      301 gtctttcagt ttcagacgca ttttgatttc acctttcaga gcaccgtctt ccgggtacat
      361 acgttcggtg gaagcttccc aacccatggt ttttttctgc ataaccggac cgtcggacgg
      421 gaagttggta ccacgcagtt taactttgta gatgaactca ccgtcttgca gggaggagtc
      481 ctgggtaacg gtaacaacac caccgtcttc gaagttcata acacgttccc atttgaaacc
      541 ttccgggaag gacagtttca ggtagtccgg gatgtcagcc gggtgtttaa cgtaagcttt
      601 ggaaccgtac tggaactgcg gggacaggat gtcccaagcg aacggcagcg gaccaccttt
      661 ggtaactttc agtttagcgg tctgggtacc ttcgtacgga cgaccttcac cttcaccttc
      721 gatttcgaac tcgtgaccgt taacggaacc ttccatacga actttgaaac gcatgaactc
      781 tttgataacg tcttcggagg aagccatcta gtatttctcc tctttctcta gtatgtgtga
      841 aa
//
