LOCUS       sequence-131461-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral vector for the expression of mouse Nkx2.2 under 
            doxycyline control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_Nkx2.2
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(847..871)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(868..906)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    918..936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    954..972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    989..1007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1025..1043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aattcctacc aagtccactg ctgggcctgg accagggggt gtgctgtcgg gtactggggg
       61 gtgctggccg agctgtactg ggcgttgtac tgcatgtgct gcagcgactg cgcgctgtag
      121 gcggaaaagg ggatgcctgc ctggaaggtg gcggctgcca ggtcctgggc tttgagcgcg
      181 tggcacggtt tgccgtccct gaccaagacc ggcactgcca cacggcgcgg cgagggcaga
      241 ggcgtcacct ccataccttt ctccgcccgg gcacgtttca tcttgtagcg atggttctgg
      301 aaccagatct tgacctgtgt cggcgtgaga cggatgaggc tggccaggtg ctcgcgctcg
      361 ggcgccgaca ggtaccgctg ctgccggaag cgccgctcca gctcgtaggt ctgcgctttg
      421 gagaagagca ctcggcgctt ccgcttcttg cctgcgtccc ccccgccgcc ctgggtctcc
      481 ttgtcattgt ccggtgactc gtcagccgag ggctctgggg acttggagct cgagtcttgg
      541 gggggagcgc tggccgccag cccgtgcagg gagtattgga ggccctcggt gctggccagc
      601 cagcgagtgt aggggttgtc gctgctgtcg tagaaagggc tcttaagggg caggctctgc
      661 acagcgtcca gggcgccctg ccccagcggc ccggccctct tggcgggctc cggcccttcg
      721 ctctcctcct ctggcccttc ggccaccgag ccgtcttcat cgttggtgtc cggaaggtcc
      781 aagatgtcct tgactgaaaa ccccgtcttt gtgttggtca gcgacatggt ggatccttct
      841 ccaggcgatc tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg
      901 cctacctcga catacgttct ctatcactga tagggagtaa actcgacata cgttctctat
      961 cactgatagg gataaactcg acatacgttc tctatcactg atagggagta aactcgacat
     1021 acgttctcta tcactgatag ggagtaaact cgaca
//
