LOCUS       sequence-131464-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral vector for the expression of mouse Olig1 under 
            doxycyline control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_Olig1
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(807..831)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(828..866)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    878..896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    914..932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    949..967
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    985..1003
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 attcctactt ggagaactgg gcctgcaccg ctgccaagcc caggccggcg gggaccagat
       61 gcgggaactt gcacaccgca caggagcaga ggccagggct acccgcgcca ccagctggga
      121 gagagaactg gccgcacggt ggctcgtcga gcgctagaga caggtacttg gtggggcgca
      181 gcgtttcggg gggtcccacg gcaccgggtg ccagcagcac agagcccggc gcggcagcca
      241 gaagaggcag gccagcgagg agcaggcgcg gggcggcggg tcccgcaccg tcgccgagcg
      301 cgcggcgcag ctcctgcagc gagctaccca gcagcaggat gtagttgcgg gcgagcagca
      361 gcgtggcaat cttggagagc ttgcggcccg gcgcgccctg gcagtgcgct gccgagtagg
      421 gtaggataac ttcgcgcagc gcgtccatgg ccaagttcag gtcctgcatg cgcttccgct
      481 cgcggctgtt gatcttgcgc cgcagctgct gctgctgctg ttcctctttg gcgtcggccc
      541 gggcgccccc ggactctggc gcaggtcccc gaggttcagc gagcggagct tcggccttct
      601 cgcgcgcagg cttggggaga agggatgcgg tggaagagga tgaggatgag gatgaggaga
      661 taggtggctg cctgtaaccc accagctcat acagcgaggc cccgagctgc acgtctccag
      721 gccgctgtgg ccgcaacatg gtcgcgggag ccgcgttcac acgcgcctgg gaaatcgcat
      781 agtacatggt ggatccttct ccaggcgatc tgacggttca ctaaacgagc tctgcttata
      841 taggcctccc accgtacacg cctacctcga catacgttct ctatcactga tagggagtaa
      901 actcgacata cgttctctat cactgatagg gataaactcg acatacgttc tctatcactg
      961 atagggagta aactcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
     1021 tctctatcac tgataggg
//
