LOCUS       sequence-131469-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV126
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..762)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
ORIGIN
        1 ggtatcgata agcttgatat cgaattcctg cagcccccag tagagaccgc ctggctctag
       61 tagcgatcta cactagcact atcagcgtta ttaagcaccg gtggagtgac gaccttcagc
      121 acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat
      181 gtcggttttg taagcacccg gcagctgaac cggtttttta gccatgtagg tggttttaac
      241 ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc attttgattt cacctttcag
      301 agcaccgtct tccgggtaca tacgttcggt ggaagcttcc caacccatgg tttttttctg
      361 cataaccgga ccgtcggacg ggaagttggt accacgcagt ttaactttgt agatgaactc
      421 accgtcttgc agggaggagt cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat
      481 aacacgttcc catttgaaac cttccgggaa ggacagtttc aggtagtccg ggatgtcagc
      541 cgggtgttta acgtaagctt tggaaccgta ctggaactgc ggggacagga tgtcccaagc
      601 gaacggcagc ggaccacctt tggtaacttt cagtttagcg gtctgggtac cttcgtacgg
      661 acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg
      721 aactttgaaa cgcatgaact ctttgataac gtcttcggag gaa
//
