LOCUS       sequence-13148-e        3495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1816-HQ858792
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1052..1151)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1168..1187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1169..1187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1192..1208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1192..1208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1310..1329)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1321..2130
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1388..1407)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2277..2865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2766..2785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3019..3036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3195..3222)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3314..3400)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3449..3471
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3463..3480
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3464..3480
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggccgtg gacctgatgg gctgctacgc cccgcgccgc
      181 gccaacgacc agctcgccat ccaggaggcg gcggcggccg ggctccgcaa cctggagctg
      241 ctggtgacgt ccctgtccac gcaggccgcc gcgccgcaca gagccgctga tcagccgttc
      301 ggcgagatcg ccggccaggc cgtctccaag ttccgcaagg tcatctccat cctcgaccgc
      361 acggggcacg cccgcttccg ccgcgggccc gtcgagccgc cgccgccgac gccgccgccg
      421 cctcctgtcg tccccggtcc tgcccccctg gcggccgtca gcgtggcgca gccgccgcag
      481 agcctgacgc tggacttcac gaagccgaac ctggccgtgt cggccgccac gtccgtcacc
      541 tccacgtcct tcttctcgtc ggtcacggcc ggcgagggca gcgtctccaa gggccggagc
      601 ctcatgtcct ccgggaagcc gccgctgtcc ggccacaagc ggaagccctg cgccggcgcc
      661 cactccgagg ccaccaccaa cggcagccgt tgccactgct ccaagagaag gaagaaccgc
      721 gtgaagagga ccatcagagt gccggcgatc agctcgaagg tcgccgacat cccttcggac
      781 gagtactcgt ggaggaagta cggccagaag cccatcaagg gctcccctta cccacggggc
      841 tactacaagt gcagcactgt gcggggatgc ccggcgagga agcacgtgga gcgggccacc
      901 gacgacccgg ccatgctggt ggtgacgtac gagggcgagc accgccacac gccgggcgcg
      961 gtccaggggc cgagccccct ggcgaccgcg tcgccggtgc ccgtcgccgt ctccgccggc
     1021 aacgggctcg ttgtcaaggg tgggcgcgcc gacccagctt tcttgtacaa agttggcatt
     1081 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1141 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1201 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1261 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1321 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1381 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1441 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1501 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1561 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1621 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1681 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1741 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1801 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1861 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1921 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1981 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2041 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2101 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2161 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2221 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2281 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2341 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2401 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2461 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2521 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2581 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2641 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     2701 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2761 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2821 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2881 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2941 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3001 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3061 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3121 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3181 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3241 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3301 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3361 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3421 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     3481 cttaagctcg ggccc
//
