LOCUS       sequence-131485-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal kinase domain of Chk1 with C-term GFP 
            fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Zhang M, Kothari P, Mullins M, Lampson MA
  TITLE     Regulation of zygotic genome activation and DNA damage checkpoint 
            acquisition at the mid-blastula transition.
  JOURNAL   Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066.
  PUBMED    25558827
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2014"; volume: "13(24):3828-38. doi"; pages: " 
            10.4161/15384101.2014.967066"
COMMENT     Sequence Label: pMZ039
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(365..383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gctcaccatc tcgaggtcag agatcttcag attatcttta tcatcaagaa gaatattctc
       61 aggctttatg tcacgatgtg taatcccaac actgtgccca aagaaacgta caatgttggg
      121 gtgtgaaagc atcttgcata tgcagacctc cttcttcaca ttctcgatgc aatccttggc
      181 ttttgccatg tccacaactt tcaccgccac agcctcttct gttttcttgt tgaccagcag
      241 tcgcacctct ccatatgctc cctctccaag agtttgtacc acatcccagt ctttaacaaa
      301 aggcacagcc atggtcctga attcgaatcg atgggatcct gcaaaaagaa caagtagctt
      361 gtattctata gtgtcaccta aatcaagctt gctccgacgt ccccaggcag aatggcggtt
      421 ccctaaacga gcattgctta tatagacctc ccattaggca cgcctaccgc ccatttacgt
      481 caatggaacg cccatttgcg tcattgcccc tccccattga cgtcaatggg gatgtacttg
      541 gcagccatcg cgggccattt accgccattg acgtcaatgg gagtactgcc aatgtaccct
      601 ggcgtacttc caatagtaat gtacttgcca agttactatt aatagatatt gatgtactgc
      661 caagtgggcc atttaccgtc attgacgtca ataggg
//
