LOCUS       sequence-131486-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Prickett TD, Wei X, Cardenas-Navia I, Teer JK, Lin JC, Walia V, 
            Gartner J, Jiang J, Cherukuri PF, Molinolo A, Davies MA, Gershenwald
            JE, Stemke-Hale K, Rosenberg SA, Margulies EH, Samuels Y
  TITLE     Exon capture analysis of G protein-coupled receptors identifies 
            activating mutations in GRM3 in melanoma.
  JOURNAL   Nat Genet. 2011 Sep 25;43(11):1119-26. doi: 10.1038/ng.950.
  PUBMED    21946352
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-09-25- 25"; volume: "43"; 
            issue: "11"; pages: "1119-26"
COMMENT     Sequence Label: pCDF1-GRM3 WT
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             440..463
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(562..579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    568..792
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(925..944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agattccagc atgttgatct ctcttaccta cgatgtgatc ctggtgatct tatgcactgt
       61 gtacgccttc aaaacgcgga agtgcccaga aaatttcaac gaagctaagt tcataggttt
      121 taccatgtac accacgtgca tcatctggtt ggccttcctc cctatatttt atgtgacatc
      181 aagtgactac agagtgcaga cgacaaccat gtgcatctct gtcagcctga gtggctttgt
      241 ggtcttgggc tgtttgtttg cacccaaggt tcacatcatc ctgtttcaac cccagaagaa
      301 tgttgtcaca cacagactgc acctcaacag gttcagtgtc agtggaactg ggaccacata
      361 ctctcagtcc tctgcaagca cgtatgtgcc aacggtgtgc aatgggcggg aagtcctcga
      421 ctccaccacc tcatctctgg attacaagga tgacgacgat aagtaggcgg ccgccactgt
      481 gctggatatc tgcagaattc caccacactg gactagtgga tccgagctcg gtaccaagct
      541 taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      601 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      661 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
      721 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
      781 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
      841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      961 tcgccggctt ttccccgtca agctctaaat cggggggctc ccttagggtt ccgatttagt
     1021 gctttacggc acctcgaccc caaaaa
//
