LOCUS       sequence-13149-e        3420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1854-HQ858793
FEATURES             Location/Qualifiers
     source          1..3420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(977..1076)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1093..1112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1094..1112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1117..1133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1117..1133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1235..1254)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1246..2055
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1313..1332)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2202..2790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2691..2710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2944..2961
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3120..3147)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3239..3325)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3374..3396
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3388..3405
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3389..3405
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggggagg tcgccgtgct gcgagaaggc gcacaccaac
      181 aagggcgcgt ggaccaagga ggaggacgag cgcctggtcg cgcacatcag ggcgcacggc
      241 gaggggtgct ggcgctcgct gcccaaggcc gccggcctcc tgcgctgcgg caagagctgc
      301 cgcctccgct ggatcaacta cctccgcccc gacctcaagc gcggcaactt cacggaggag
      361 gaggacgagc tcatcgtcaa gctgcacagc gtcctcggca acaagtggtc cctgatcgcc
      421 ggaaggctgc ccggcaggac ggacaacgag atcaagaact actggaacac gcacatccgg
      481 aggaagctgc tgagcagggg gatcgacccg gtgacgcacc gcccggtcac ggagcaccac
      541 gcgtccaaca tcaccatatc gttcgagacg gaggtggccg ccgctgcccg tgatgataag
      601 aagggcgccg tcttccggct ggaggaggag gaggagcgca acaaggcgac gatggtcgtc
      661 ggccgcgacc ggcagagcca gagccagagc cacagccacc ccgccggcga gtggggccag
      721 gggaagaggc cgctcaagtg ccccgacctc aacctggacc tctgcatcag cccgccgtgc
      781 caggaggagg aggagatgga ggaggctgcg atgagagtga gaccggcggt gaagcgggag
      841 gccgggctct gcttcggctg cagcctgggg ctccccagga ccgcggactg caagtgcagc
      901 agcagcagct tcctcgggct caggaccgcc atgctcgact tcagaagcct cgagatgaaa
      961 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1021 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1081 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1141 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1201 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1261 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1321 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1381 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1441 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1501 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1561 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1621 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1681 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1741 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1801 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1861 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1921 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1981 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2041 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2101 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2341 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2581 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2641 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2701 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2761 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2821 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2881 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2941 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3001 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3061 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3121 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3181 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3241 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3301 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3361 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
