LOCUS       sequence-131491-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Prickett TD, Wei X, Cardenas-Navia I, Teer JK, Lin JC, Walia V, 
            Gartner J, Jiang J, Cherukuri PF, Molinolo A, Davies MA, Gershenwald
            JE, Stemke-Hale K, Rosenberg SA, Margulies EH, Samuels Y
  TITLE     Exon capture analysis of G protein-coupled receptors identifies 
            activating mutations in GRM3 in melanoma.
  JOURNAL   Nat Genet. 2011 Sep 25;43(11):1119-26. doi: 10.1038/ng.950.
  PUBMED    21946352
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-09-25- 25"; volume: "43"; 
            issue: "11"; pages: "1119-26"
COMMENT     Sequence Label: pCDF1-GRM3 (E870K)
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             439..462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        507..718
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             731..1000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 gattccagca tgttgatctc tcttacctac gatgtgatcc tggtgatctt atgcactgtg
       61 tacgccttca aaacgcggaa gtgcccagaa aatttcaacg aagctaagtt cataggtttt
      121 accatgtaca ccacgtgcat catctggttg gccttcctcc ctatatttta tgtgacatca
      181 agtgactaca gagtgcagac gacaaccatg tgcatctctg tcagcctgag tggctttgtg
      241 gtcttgggct gtttgtttgc acccaaggtt cacatcatcc tgtttcaacc ccagaagaat
      301 gttgtcacac acagactgca cctcaacagg ttcagtgtca gtggaactgg gaccacatac
      361 tctcagtcct ctgcaagcac gtatgtgcca acggtgtgca atgggcggaa agtcctcgac
      421 tccaccacct catctctgga ttacaaggat gacgacgata agtaggcggc cgcgaaggat
      481 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      541 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
      601 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
      661 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
      721 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
      781 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
      841 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
      901 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
      961 cgtctttgtt tccgttttct gttctgcgcc gttacaaatc caagctgtga ccgg
//
