LOCUS       sequence-13150-e        3411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1855-HQ858794
FEATURES             Location/Qualifiers
     source          1..3411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(968..1067)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1084..1103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1085..1103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1108..1124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1108..1124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1226..1245)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1237..2046
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1304..1323)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2193..2781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2682..2701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2935..2952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3111..3138)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3230..3316)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3365..3387
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3379..3396
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3380..3396
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggttcca tccgagatgg ggagcgtcgc caccgccgtc
      181 agcacgcctg ctgcggataa gctgctccat gggcctatct ctggcaagaa gtgcaagaaa
      241 ccaggcccga ggaagattca taaggctgag agggagaagc ttaaacgaga ccacctaaat
      301 gatctctttg ttgagctggg taatatgcta gaagcagata gacagaacaa tgggaaagca
      361 tgcatactga ctgacactac tcggatacta agagacctgc ttgttcaagt agactctctt
      421 cgaaaggaac atagcgacct acaaaacgaa tctcattatg taacaatgga gagggatgag
      481 atgcaggatg aaaacggcgt gctccgcaaa gaaatttcag agcttcaaaa cgagttgaca
      541 atgcgggttt caggcagtcc agcaggttgg ggccatggca ccgccagatc agatccccct
      601 cttcctcact caaccgcagt gttctcgtcg gagcaggcga tgcaaccacc caccattgca
      661 agcgttgtat tcccgttgca gcagccactg gcaccatcag caatgaccaa accacctcat
      721 gctgcaccac cactcgaact gaagctcttt ccagaagtgg catctgccga aggccatgag
      781 ccgtgtgaag accacaaagc tcccaaccat gtggcacgac cacaggcaag gtacccaacg
      841 caatcagcct catggcccgt aaacctgttc tctggccttc cacgaatgga agatgagcaa
      901 tgcagtagca gcacaagcgg cagcagcaga gaggataaca cagatataga aaagggtggg
      961 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1021 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1081 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1141 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1201 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1261 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1321 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1381 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1441 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1501 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1561 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1621 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1681 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1741 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1801 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1861 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1921 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     1981 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2041 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2101 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2161 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2221 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2281 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2341 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2401 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2461 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2521 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2581 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2641 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2701 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2761 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2821 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2881 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2941 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3001 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3061 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3121 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3181 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3241 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3301 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3361 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
