LOCUS       sequence-131509-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses ER-retained GCaMP3 under 
            control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..531)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     647..666
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcgg
       61 cgcgccctac aactcatctt tttctgctgt cttcgctgtc atcatttgta caaactcttc
      121 gtagtttacc tgaccatccc catcgatgtc tgcttccctg atcatttcat caacctcttc
      181 atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg cactgatgta
      241 gccattgcca tccttatcaa acacacggaa cgcttctcta atttcttctt cactgtctgt
      301 gtctttcatt tttcttgcca tcattgtcag gaactcaggg aagtcgattg tgccgtcacc
      361 gtcggcatct acttcattga tcatgtcctg cagctctgct tctgtggggt tctgccccag
      421 agaccgcatc accgtcccca gctccttggt tgttattgtc ccatccccgt ccttgtcaaa
      481 tagggagaaa gcctctttaa attctgcgat ctgctcttca gtcagttggt cacgcgtgtt
      541 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      601 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      661 gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct tcgggcatgg cggacttgaa
      721 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
      781 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      841 ggtcagcttg ccgtaggtgg catcgccctc accctcgccg gacacgctga acttg
//
