LOCUS       sequence-13151-e        3777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1856-HQ858795
FEATURES             Location/Qualifiers
     source          1..3777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1334..1433)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1450..1469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1451..1469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1474..1490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1474..1490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1592..1611)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1603..2412
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1670..1689)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2559..3147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3048..3067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3301..3318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3477..3504)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3596..3682)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3731..3753
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3745..3762
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3746..3762
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggacgac gacgacgacg tgtcgccctc ctcctccccg
      181 tcgccgccct cctcctccgc gctccccgtg gacggccccg tcaccgtcgc cgctgcgccg
      241 ccgggcgctt ccatggcggt cgcgctcccg atccatagga ccgcggcgtc cctgtacgct
      301 agcgccggcg gcgatggcgg cggcggcggc gggagggagg acgcctggag cgacggcgcc
      361 acgtccgcgc tcatcgacgc ctggggcgag cgcttcgtcg cgctcggccg cggcagcctc
      421 cgccacccgc agtggcagga ggtcgccgaa gccgtctcct cccgtgacgg ctactccaag
      481 gcgcccaagt ccgatatcca gtgcaaaaac cgcatcgaca cgctcaagaa gaagtacaag
      541 gtcgagagga tcaagccggt ctccggctgg cagttcttcg accgcctcga ttttctcctc
      601 gcgcccacct acggcaacaa gcccggcaac ggcggcggcg ggcacaactc caacagccgc
      661 agccagatgc ctgcggcgcc gcgagtgggc ttccctcaac gcagccgcac gccgctcatg
      721 cccgcagcag gatccggggc caagcggagg gcgccttcgc cggagccgtc ggtgtcctcc
      781 gattcctccg acggcttccc gccaatgccg gcccttccgg cggtgaacgg gaagaggaag
      841 aggacggacg atgggcgctc cgacggaggc ggcgaccgcg cgcaggggct tcgggagctg
      901 gctcaggcga tacggcgcct cggcgaggcg tacgagcgcg tggaggccac gaagctggaa
      961 caggaggccg agatggagcg tcggcgcatg gacttcgcgc aggagctgga gtcgcagcgc
     1021 gtgcggttct tcctcgacac gcagatggag ctcacgcagg ccaagaatca cgcgtctcct
     1081 gccacggccg ccgtccctgc tgttggctcg tcgaggagga ttccgaggat gtcactggtt
     1141 accgacgccg gtggcagcag caatcaccat agccgttacc ggatcagcca tgttgacagg
     1201 caccgccatg ctccacgccc acattaccag cagtaccatg acaacaataa cgcgggggca
     1261 gcggctgcaa gtgacggtga gcagtctgac gaggaagatt atgaagaaga gagccagaag
     1321 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1381 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1441 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1501 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1561 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1621 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1681 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1741 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1801 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1861 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1921 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1981 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2041 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2101 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2161 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2221 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2281 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2341 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2401 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2461 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2701 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2941 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3001 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3061 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3241 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3301 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3361 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3421 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3481 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3541 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3601 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3661 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3721 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
