LOCUS       sequence-131516-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses ER-retained low-affinity 
            GCaMP3 variant under control of the SYN1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3
            (D324G+D...
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..162
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(166..174,175..189)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      196..205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             217..273
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     486..507
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(613..634)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcgtgcctg agagcgcagt cgagaaggta ccggatccgc tagccaccat gctgctatcc
       61 gtgccgttgc tgctcggcct cctcggcctg gccgtcgcca tgggttctca tcatcatcat
      121 catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat
      181 gacgataagg atctcgccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt
      241 cacgcagtca gagctatagg tcggctgagc tcactcgaga acgtctatat caaggccgac
      301 aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcggc
      361 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      421 cccgacaacc actacctgag cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc
      481 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      541 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
      601 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      661 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      721 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      781 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      841 ggctacatcc aggagcgcac catcttcttc aaggacgacg gca
//
