LOCUS       sequence-131525-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of 
            ML1N*2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: p3E-ML1N*2
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..114
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    584..708
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(779..796)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(779..795)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa gttgttcgga
      121 tccttaatta tccaggtcag gggggtccag catcagttta catggcttgc gccctttggc
      181 ccgaaattta tcgcaagggc tcataaagaa atacttcagc cgccttctca ggtcttcctc
      241 ttcggtaggg gtagtaggtg ctggggatgt tccgacctgg gttccgtagc ctgggttagg
      301 ggtcagcagt ctttcagtct cgcttgctcg tcgtccagct ggagttgcca ttgtcaggat
      361 tctcagcatc agcttacagg gttttcgccc ctttgcgcgg aatttatcgc atggactcat
      421 aaagaagtac ttcagccgtc tcctcaggtc ctcttcctca gtgggtgtgg tgggagctgg
      481 agatgtgccc acttgagtgc cgtatccggg gttgggtgtc agcagccgtt cggtctcgct
      541 tgccctccgt ccagcaggtg ttgccatagt caggtcagag tacaccactt tgtacaagaa
      601 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      661 aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa ccaactactt
      721 agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcccg agcttaagac
      781 tggccgtcgt tttacaacgt cgtgactgg
//
