LOCUS       sequence-131531-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein with mutated 
            (D10A, H840A) and inactivated catalytic domains as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pD10AH840AhCas9 (GB1041)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(89..100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt cattttcttg
       61 acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga tcttgtcaac
      121 actttattat caatagaatc atctttgaga aaagactggg gcacgatggc atccacgtcg
      181 tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct gccgttctgc
      241 aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg gtgttcctta
      301 aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct ttccctactg
      361 ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac gatattctcg
      421 ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt ctgcagtatt
      481 ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtggag actgtccccc
      541 tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc atcatggatc
      601 aactgcatga agttccggtt ggcaaatcca tcggacttaa gaaaatccag gattgtcttt
      661 ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc ccatcctgta
      721 tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga agagatgagc gtaagttttc
      781 aagcgttctt caatcatctc cctatcttca a
//
