LOCUS       sequence-131538-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Cas9 in plants, cauliflower mosaic virus 35S promoter 
            (P35S), hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLCRISPR/Cas9P35S-H
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(127..145)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(128..145)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    187..203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(211..217,218..235,236..241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    256..277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgatgttcg
       61 agcggtcgcc agtgtgatgg gtatctacag actagaccca ttgagcttga tgcatagctt
      121 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcttgt
      181 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      241 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      301 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccact tcagaggtct
      361 ctcgagcact acggcgcgcc ttaccggtca acatgtggag cacgacacac ttgtctactc
      421 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
      481 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
      541 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
      601 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
      661 cgtggaaa
//
