LOCUS       sequence-131556-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constiutive mammalian expression of the bioluminescent GpNLuc 
            LumiFluor reporter for single-cell analysis and sensitive optical 
            imaging of living subjects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, 
            Cleveland JL, Amelio AL
  TITLE     Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical 
            Imaging and Flow Cytometry for Monitoring Tumorigenesis.
  JOURNAL   Cancer Res. 2015 Sep 30. pii: canres.3538.2014.
  PUBMED    26424696
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Sep 30. pii: canres.3538.2014."
COMMENT     Sequence Label: pRetroX-Tight-MCS_PGK-GpNLuc
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     363..386
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(480..504)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(501..539)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    551..569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    587..605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    622..640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    658..676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    694..712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
       61 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
      121 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
      181 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
      241 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
      301 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
      361 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
      421 cggtagaatt cacgcgttcg cgatctagag ccggcgcggc cgcggatcct tctccaggcg
      481 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
      541 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
      601 agggataaac tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
      661 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaa
//
