LOCUS       sequence-131557-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constiutive mammalian expression of the bioluminescent OgNLuc 
            LumiFluor reporter for single-cell analysis and sensitive optical 
            imaging of living subjects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, 
            Cleveland JL, Amelio AL
  TITLE     Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical 
            Imaging and Flow Cytometry for Monitoring Tumorigenesis.
  JOURNAL   Cancer Res. 2015 Sep 30. pii: canres.3538.2014.
  PUBMED    26424696
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Sep 30. pii: canres.3538.2014."
COMMENT     Sequence Label: pRetroX-Tight-MCS_PGK-OgNLuc
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(26..45)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     419..442
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(533..847)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(536..560)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    643..661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    678..696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    714..732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    750..768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    785..803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    821..839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg
       61 accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc
      121 ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa
      181 ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg
      241 ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga
      301 ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc
      361 taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca
      421 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
      481 agaattcacg cgttcgcgat ctagagccgg cgcggccgcg gatccttctc caggcgatct
      541 gacggttcac taaacgagct ctgcttatat aggcctccca ccgtacacgc ctacctcgac
      601 atacgttctc tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg
      661 ataaactcga catacgttct ctatcactga tagggagtaa actcgacata cgttctctat
      721 cactgatagg gagtaaactc gacatacgtt ctctatcact gatagggagt aaactcgaca
      781 tcgttctcta tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag
      841 taaactcgag tgaagacgaa agggcctcag atcttcaatt ccggcgccta
//
