LOCUS       sequence-13156-e        3657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1887-HQ858800
FEATURES             Location/Qualifiers
     source          1..3657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1214..1313)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1330..1349)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1331..1349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1354..1370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1354..1370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1472..1491)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1483..2292
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1550..1569)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2928..2947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3181..3198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3357..3384)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3476..3562)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3611..3633
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3625..3642
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3626..3642
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgaaggtg ctctgctccg cgtgcgaggc ggccgaggcg
      181 cgcctgctct gctgcgccga cgaggctgcc ctctgcgcgc gctgcgaccg cgacgtgcac
      241 gccgccaacc gcctcgccgg caagcaccac cgcctgccgc tcctcccgcc cgacgacgtc
      301 tccgcgccca actgcgacat atgccaggag gcccacgcct acttcttctg cgtggaggac
      361 cgcgcgctgc tctgccgggc ctgcgacatg gccgtgcaca cggccaacgc cttcgtctcc
      421 gcgcaccgca ggttcctcct caccggcgtc caggtcggcc tgcagcccgc cgccgcggcc
      481 caggacgcag acccgcaccc gcccgccgcc gcggaacccc tccagacgcc cccgccgccc
      541 gacaggaagg cggcggcggc cggcgacggc agcagcccgg cgctgctcta cagcgacgac
      601 gacatcgact gggcggccgg cgcggacgcc ggcggcggcg tcagcgtcgc cgtcaccctg
      661 ccggactggt cgctcgtcca cgagcagttc ggcgccccgc cggtggtgcc ccgacacgcg
      721 gacccggcgc tagccagaac gccctcttcc aagcgcagtc cccggcggtc ggtcgccgcg
      781 gcgttcaccg tccagggcgg cggcggcctt gcagggggct tgccggactg gccgctcgac
      841 gagttctttg gcttctccga gtacagcgcc ggcctcgcct tcgctgagaa tgacacgtcc
      901 aaggccgaca gcgggaagct cgggagcacc gacggctcgc cggccggccg gccgtcgtcc
      961 gacgcgtccc aggacttctt cgggcaggtg ccggagttcc accagtggtc cgtgccggag
     1021 ctcccttccc cgcccacggc ctcggggctc cactggcagg gcggcccgcg ccacggcgcg
     1081 gccaccacca ccgacgtggc cgccgtgttc gtgccggaca tctcgtcccc ggagaacccc
     1141 ttctggtgct acgccaccgc cgctggccag gcgccgacga agcgccgcag gagatgtaag
     1201 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1261 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1321 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1381 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1441 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1501 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1561 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1621 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1681 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1741 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1801 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1861 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1921 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1981 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2041 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2101 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2161 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2221 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2281 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2341 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3181 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3241 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3301 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3361 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3421 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3481 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3541 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3601 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
