LOCUS       sequence-131585-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP64 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC152-dual-dCas9VP64-sgExpression
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..190)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgaaatcgtc cagagcatcg gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc
       61 ttcccaacat atccaggtca aagtcatcga gggcatcaga acccagcatg tcgagatcga
      121 aatcgtccag cgcgtcggcg cgcccggatc cggccggccc gctggcctcc acctttctct
      181 tcttcttggg gctgtcgcct cccagctgag acaggtcgat ccgtgtctcg tacaggccgg
      241 tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc
      301 ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg gtcagggtaa
      361 acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc ttgttgtagg
      421 cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag aactcgctga
      481 tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc tgtttctgct
      541 cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac aggaagttca
      601 catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca gaggccagca
      661 ttctcttccg gccgttttcc agctcgaaca gggagtactt
//
