LOCUS       sequence-13160-e        3729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1182-HQ858804
FEATURES             Location/Qualifiers
     source          1..3729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1286..1385)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1402..1421)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1403..1421)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1426..1442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1426..1442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1544..1563)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1555..2364
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1622..1641)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2511..3099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3000..3019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3253..3270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3429..3456)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3548..3634)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3683..3705
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3697..3714
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3698..3714
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggatggtg cttcctccat tagtcctgca tttacttcaa attatcagca aagtaagctg
      181 gcagatccaa tccaatcaaa accgaagccc cttaccccaa aggatttaat gcctgtccaa
      241 tttagttcgt cgctgcctga tcctacctca atctgttttg agggtatagc tgccataact
      301 acacccaatt ttgaggatca tggcaacaga gccagcattg cttcaggggc aaaagatgtg
      361 aacatgttcc caacatttaa agaccagtcc agtaatcgtc atgatgcact gttttctaat
      421 gtcgatgggt acatgcaaca gagacgaaat aatcactttg gaactgaagt atgtggctta
      481 tctgaaagca caggcatttc ccagtcggac cagttcacag tatcgaaatt ccagaatggc
      541 aggcccagca gcttgggcct ttcgaatgga aatcttgggg ttctatcgac tgggagaaga
      601 gaggaagcac gtgaaggctt gtttaggcct tgttctgtca agacaagtgc tgggaatgag
      661 gagcagcaaa agcggcctgg tgatgtcaag ttgtttgggc aaattcttag ccatcaatca
      721 tccttgcaaa gctctggttc atcagttcat ggaagcaaaa gcaagccccc ctcaccaaag
      781 gttgataagc cagcttcttc taggttgttg agtaatccaa gggaacgcct ggtttattct
      841 tccaggcctc caatcattgc taacttgggg ctagaggaga gagcgatgag gagttttgac
      901 catatggatg ggcgaacaat acagcctgag cctatggtta tggtggcaaa gtgtcagaga
      961 ccgtcagctg gtgtaccagt ttactcaacc aaaaatggcg cgctcagcgt gtttgcggaa
     1021 tttcagcaac cttcaatgcc gccacataca tcagatcaca aactactaga aaactttgcc
     1081 gatcttcata agaggaatgg aatcgaactc ctttcggggt tccagcagcc tgggaggctt
     1141 gggggtgctg gggtcctggt tagcggtgta tctgaccctg tggctgctct caaggcacag
     1201 tacggctctg gttcgaagat gttgagcagc agcaacgacg tggatacatg gaaagacata
     1261 ggaagcagga agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga
     1321 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1381 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1441 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1501 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1561 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1621 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1681 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1741 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1801 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     1861 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1921 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     1981 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2041 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2101 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2161 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2221 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2281 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2341 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2401 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2461 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2521 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2581 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2641 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     2701 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2761 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2821 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2881 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2941 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3001 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3061 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3121 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3181 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3241 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3301 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3361 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3421 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3481 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3541 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3601 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3661 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     3721 ctcgggccc
//
