LOCUS       sequence-13161-e        3393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1183-HQ858805
FEATURES             Location/Qualifiers
     source          1..3393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(950..1049)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1066..1085)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1067..1085)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1090..1106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1090..1106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1208..1227)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1219..2028
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1286..1305)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2175..2763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2664..2683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2917..2934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3093..3120)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3212..3298)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3347..3369
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3361..3378
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3362..3378
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgaagatcc agtgcgacgc gtgcgagagc gcggcggcgg cggtggtgtg ctgcgcggac
      181 gaggcggcgc tgtgcgcggc gtgcgacgtg gaggtgcacg cggcgaacaa gctggccggg
      241 aagcaccagc ggctgccgct ggaggcgctc tcggcgaggc tcccgcgctg cgacgtgtgc
      301 caggagaagg cggcgttcat cttctgcgtg gaggaccgcg cgctcttctg ccgcgactgc
      361 gacgagccca tccacgtccc cggcacgctc tccggcaacc accagcgcta cctcgccacc
      421 ggcatccgcg tcggcttcgc ctccgcctcg ccctgcgacg gcggcagcga cgcccatgac
      481 tccgaccacc acgccccgcc catgggctcc tccgagcatc atcaccatca tcagcagccg
      541 gccccgaccg tcgccgtcga cacgccctcg ccgcagttcc tgccgcaggg ctgggccgtc
      601 gacgagctcc tccagttctc cgactacgag accggcgaca agctgcagaa ggagtcgtcg
      661 ccgccgctcg ggttccagga gctggagtgg ttcgccgaca tcgacctgtt ccacaaccag
      721 gcgcccaagg gcggcgccgc cgccggccgg acgacggcgg aggtccccga gctcttcgct
      781 tcgcaggcgg ccaacgacgt ggcgtactac aggccgccga ccaggaccgc cgccgccgcc
      841 ttcaccgcgg ccaccggctt ccgccagagc aagaaggccc gcgtcgagct ccccgacgac
      901 gaggaggatt acctcatcgt ccctgatctt ggtaagggtg ggcgcgccga cccagctttc
      961 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1021 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1081 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1141 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1201 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1261 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1321 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1381 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1441 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1501 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     1561 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1621 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1681 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1741 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     1801 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     1861 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     1921 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     1981 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2041 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2101 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2281 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     2581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2881 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2941 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3001 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3061 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3121 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3181 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3241 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3301 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     3361 tgtaaaacga cggccagtct taagctcggg ccc
//
